English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Deep tissue two-photon microscopy

Helmchen, F., & Denk, W. (2005). Deep tissue two-photon microscopy. Nature methods, 2(12), 932-940. doi:10.1038/nmeth818.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-9B52-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-BC00-7
Genre: Journal Article
Alternative Title : Deep tissue two-photon microscopy

Files

show Files
hide Files
:
6528.pdf (Any fulltext), 576KB
 
File Permalink:
-
Name:
6528.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute of Neurobiology, MMNB; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: INSTITUT
License:
-

Locators

show
hide
Description:
-
Locator:
http://dx.doi.org/10.1038/nmeth818 (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Helmchen, Fritjof1, Author              
Denk, Winfried2, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, Jahnstrasse 29, 69120 Heidelberg, DE, ou_1497701              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

Content

show
hide
Free keywords: -
 Abstract: With few exceptions biological tissues strongly scatter light, making high−resolution deep imaging impossible for traditional−including confocal−fluorescence microscopy. Nonlinear optical microscopy, in particular two photon−excited fluorescence microscopy, has overcome this limitation, providing large depth penetration mainly because even multiply scattered signal photons can be assigned to their origin as the result of localized nonlinear signal generation. Two−photon microscopy thus allows cellular imaging several hundred microns deep in various organs of living animals. Here we review fundamental concepts of nonlinear microscopy and discuss conditions relevant for achieving large imaging depths in intact tissue

Details

show
hide
Language(s): eng - English
 Dates: 200520052005-12-01
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature methods
  Other : Nature methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 2 (12) Sequence Number: - Start / End Page: 932 - 940 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556