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Zusammenfassung:
Multiphoton microscopy allows imaging of cellular activity in living tissue explants and in whole animals and has thus become a powerful tool for studying neuronal activity. The miniaturization of laser−scanning microscopy promises to extend activity measurements to awake, freely moving animals. Here we describe and evaluate a miniature multiphoton microscope based on a "piezolever fiber scanner" (PLFS). Our current PLFS has a scan range of 1.1 mm and a resonance frequency of 790 Hz. It allows, unlike resonance−based designs, a lateral shift of the scanned area, and offers the prospect of random−access (vector) scanning
