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  Ultrafast temporally stochastic STED nanoscopy of millisecond dynamics.

Schneider, J., Zahn, J., Maglione, M., Sigrist, S. J., Marquard, J., Chojnacki, J., et al. (2015). Ultrafast temporally stochastic STED nanoscopy of millisecond dynamics. Nature Methods, 12(9), 827-830. doi:10.1038/nmeth.3481.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-D460-F Version Permalink: http://hdl.handle.net/21.11116/0000-0002-A715-A
Genre: Journal Article

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 Creators:
Schneider, J., Author
Zahn, J., Author
Maglione, M., Author
Sigrist, S. J., Author
Marquard, J., Author
Chojnacki, J., Author
Kräusslich, H. G., Author
Sahl, S. J.1, Author              
Engelhardt, J.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Free keywords: Super-resolution microscopy, Confocal microscopy, Fluorescence imaging
 Abstract: Electro-optical scanning (>1,000 frames/s) with pixel dwell times on the order of the lifetime of the fluorescent molecular state renders stimulated emission depletion (STED) nanoscopy temporally stochastic. Photon detection from a molecule occurs stochastically in one of several scanning frames, and the spatial origin of the photon is known with subdiffraction precision. Images are built up by binning consecutive frames, making the time resolution freely adjustable. We demonstrated nanoscopy of vesicle motions in living Drosophila larvae and the cellular uptake of viral particles with 5- to 10-ms temporal resolution.

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Language(s): eng - English
 Dates: 2015-07-272015-09
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nmeth.3481
 Degree: -

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Title: Nature Methods
Source Genre: Journal
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Pages: - Volume / Issue: 12 (9) Sequence Number: - Start / End Page: 827 - 830 Identifier: -