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  The exocytotic event in chromaffin cells revealed by patch amperometry.

Albillos, A., Dernick, G., Horstmann, H., Almers, W., de Alvarez Toledo, G., & Lindau, M. (1997). The exocytotic event in chromaffin cells revealed by patch amperometry. Nature, 389, 509-512. doi:10.1038/39081.

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Albillos, A., Author
Dernick, G., Author
Horstmann, H., Author
Almers, W., Author
de Alvarez Toledo, G., Author
Lindau, M.1, Author              
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1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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 Abstract: In mast cells and granulocytes, exocytosis starts with the formation of a fusion pore. It has been suggested that neurotransmitters may be released through such a narrow pore without full fusion. However, owing to the small size of the secretory vesicles containing neurotransmitter, the properties of the fusion pore formed during Ca2+-dependent exocytosis and its role in transmitter release are still unknown. Here we investigate exocytosis of individual chromaffin granules by using cell-attached capacitance measurements combined with electrochemical detection of catecholamines, achieved by inserting a carbon-fibre electrode into the patch pipette. This allows the simultaneous determination of the opening of individual fusion pores and of the kinetics of catecholamine release from the same vesicle. We found that the fusion-pore diameter stays at <3 nm for a variable period, which can last for several seconds, before it expands. Transmitter is released much faster through this pore than in mast cells, generating a 'foot' signals which precedes the amperometric spike. Occasionally, the narrow pore forms only transiently and does not expand, allowing complete transmitter release without full fusion of the vesicle with the plasma membrane.

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Language(s): eng - English
 Dates: 1997-10-02
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/39081
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 389 Sequence Number: - Start / End Page: 509 - 512 Identifier: -