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  A novel Ca2+-dependent step in exocytosis subsequent to vesicle fusion.

Hartmann, J., & Lindau, M. (1995). A novel Ca2+-dependent step in exocytosis subsequent to vesicle fusion. FEBS Letters, 363(3), 217-220. doi:10.1016/0014-5793(95)00318-4.

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Hartmann, J., Author
Lindau, M.1, Author              
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1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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Free keywords: Exocytosis; Calcium; Eosinophil; Fusion pore
 Abstract: Exocytosis begins with formation of a small fusion pore which then expands allowing rapid release of granular contents. We studied the influence of cytoplasmic free Ca2+ ([Ca2+]i) on the conductance of the initial pore and on the dynamics of subsequent expansion in horse eosinophils using the patch clamp technique. The mean initial conductance is ∼200 pS independent of [Ca2+]i. This value is close to that previously found in beige mouse mast cells. The pore subsequently expands by 18 nS/s at [Ca2+]i < 10 nM, by 40 nS/s at [Ca2+]i = 1.5 μM and by 90 nS/s at [Ca2+]i = 10 μM. These results show that the structure of the initial fusion pore is independent of cytoplasmic Ca2+. However, the pore expansion is a Ca2+-dependent process modulating secretion at a step later than vesicle-plasma membrane fusion.

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Language(s): eng - English
 Dates: 2000-03-071995-04-24
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0014-5793(95)00318-4
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Title: FEBS Letters
Source Genre: Journal
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Pages: - Volume / Issue: 363 (3) Sequence Number: - Start / End Page: 217 - 220 Identifier: -