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  Intracellular application of guanosine-5'-O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils.

Nüsse, O., Lindau, M., Cromwell, O., Kay, A. B., & Gomperts, B. D. (1990). Intracellular application of guanosine-5'-O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils. Journal of Experimental Medicine, 171(3), 775-786. doi:10.1084/jem.171.3.775.

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2175901.pdf (Publisher version), 755KB
 
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Nüsse, O., Author
Lindau, M.1, Author           
Cromwell, O., Author
Kay, A. B., Author
Gomperts, B. D., Author
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1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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 Abstract: The mechanism of eosinophil secretion was studied in guinea pig eosinophils by measuring release of hexosaminidase from cell suspensions (greater than 98% pure) permeabilized with streptolysin-O and by whole-cell patch-clamp capacitance measurements. It is shown that release of eosinophil granule components occurs by an exocytotic mechanism in which individual granules fuse with the plasma membrane. Exocytosis can be induced by intracellular application of the nonhydrolyzable GTP analog guanosine-5'-O-(3-thiotriphosphate) (GTP-gamma-S), suggesting the involvement of a GTP-binding protein. The activation is modulated by the intracellular calcium concentration, with activation by GTP-gamma-S inducing transient elevations in the concentration of Ca2+. Thus, the nature and regulation of the release mechanism appear to be very similar to that of the mast cell and neutrophil.

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Language(s): eng - English
 Dates: 1990-03-01
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1084/jem.171.3.775
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Title: Journal of Experimental Medicine
Source Genre: Journal
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Pages: - Volume / Issue: 171 (3) Sequence Number: - Start / End Page: 775 - 786 Identifier: -