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The technology to eavesdrop on exocytosis events is improving and bringing new insights to our understanding of cellular communication. Thanks to a generous grant from the Kavli Institute at Cornell for Nanoscale Science and with support from HEKA Electronic, a workshop entitled Advanced Techniques to Study Single Vesicle Exocytosis and a more informal W&BC (wine and beer club) on Advanced Instrumentation for these studies, were held during the 13th International Symposium on Chromaffin Cell Biology in Pucón, Chile, which took place January 7–12, 2006. Ana Cárdenas of Valparaíso University organized an outstanding meeting in a spectacular environment between Lake Villarica and the impressive Villarrica Volcano. The meeting attracted 120 attendees from about 15 countries who had very productive discussions on many topics related to exocytosis, ranging from molecular to clinical aspects.