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  Chemotaxis of Dictyostelium discoideum: Collective Oscillation of Cellular Contacts

Schäfer, E., Tarantola, M., Polo, E., Westendorf, C., Oikawa, N., Bodenschatz, E., et al. (2013). Chemotaxis of Dictyostelium discoideum: Collective Oscillation of Cellular Contacts. PLoS ONE, 8(1), e54172-1-e54172-12. doi:10.1371/journal.pone.0054172.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-1035-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-1036-4
Genre: Journal Article

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 Creators:
Schäfer, Edith, Author
Tarantola, Marco1, Author              
Polo, Elena, Author
Westendorf, Christian1, Author              
Oikawa, Noriko1, Author              
Bodenschatz, Eberhard1, Author              
Geil, Burkhard, Author
Janshoff, Andreas, Author
Affiliations:
1Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063287              

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 Abstract: Chemotactic responses of Dictyostelium discoideum cells to periodic self-generated signals of extracellular cAMP comprise a large number of intricate morphological changes on different length scales. Here, we scrutinized chemotaxis of single Dictyostelium discoideum cells under conditions of starvation using a variety of optical, electrical and acoustic methods. Amebas were seeded on gold electrodes displaying impedance oscillations that were simultaneously analyzed by optical video microscopy to relate synchronous changes in cell density, morphology, and distance from the surface to the transient impedance signal. We found that starved amebas periodically reduce their overall distance from the surface producing a larger impedance and higher total fluorescence intensity in total internal reflection fluorescence microscopy. Therefore, we propose that the dominant sources of the observed impedance oscillations observed on electric cell-substrate impedance sensing electrodes are periodic changes of the overall cell-substrate distance of a cell. These synchronous changes of the cell-electrode distance were also observed in the oscillating signal of acoustic resonators covered with amebas. We also found that periodic cell-cell aggregation into transient clusters correlates with changes in the cell-substrate distance and might also contribute to the impedance signal. It turned out that cell-cell contacts as well as cell-substrate contacts form synchronously during chemotaxis of Dictyostelium discoideum cells.

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Language(s): eng - English
 Dates: 2013-01-17
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 649918
DOI: 10.1371/journal.pone.0054172
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Title: PLoS ONE
Source Genre: Journal
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Pages: - Volume / Issue: 8 (1) Sequence Number: - Start / End Page: e54172-1 - e54172-12 Identifier: -