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  Live cell flattening - traditional and novel approaches

Westendorf, C., Bae, A., Erlenkamper, C., Galland, E., Franck, C., Bodenschatz, E., et al. (2010). Live cell flattening - traditional and novel approaches. PMC Biophysics, 3, 9-1-9-15. doi:10.1186/1757-5036-3-9.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-126F-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-1270-E
Genre: Journal Article

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 Creators:
Westendorf, Christian1, Author              
Bae, Albert1, Author              
Erlenkamper, Christoph, Author
Galland, Edouard, Author
Franck, Carl, Author
Bodenschatz, Eberhard1, Author              
Beta, Carsten1, Author              
Affiliations:
1Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063287              

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 Abstract: Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method.

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Language(s): eng - English
 Dates: 2010-04-19
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 476830
DOI: 10.1186/1757-5036-3-9
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Title: PMC Biophysics
  Alternative Title : PMC Biophys.
Source Genre: Journal
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Pages: - Volume / Issue: 3 Sequence Number: - Start / End Page: 9-1 - 9-15 Identifier: -