English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Highly Packed and Oriented DNA Mesophases Identified Using in Situ Microfluidic X-ray Microdiffraction

Pfohl, T., Otten, A., Köster, S., Dootz, R., Struth, B., & Evans, H. M. (2007). Highly Packed and Oriented DNA Mesophases Identified Using in Situ Microfluidic X-ray Microdiffraction. Biomacromolecules, 8(7), 2167-2172. doi:10.1021/bm070317s.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-1419-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-141A-6
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Pfohl, Thomas1, Author              
Otten, Alexander, Author
Köster, Sarah1, Author              
Dootz, Rolf1, Author              
Struth, Bernd, Author
Evans, Heather M.1, Author              
Affiliations:
1Group Dynamics of biological matter, Department of Dynamics of Complex Fluids, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063313              

Content

show
hide
Free keywords: -
 Abstract: DNA condensation in vivo usually requires proteins and/or multivalent salts. Here, we explore the in vitro compaction of DNA by cationic dendrimers having an intermediate size and charge. The dynamic assembly of DNA-dendrimer mesophases is discernible due to the laminar flow in a specially designed X-ray compatible microfluidic device. The setup ensures a nonequilibrium ascent of reactant concentration, and the resulting progression of DNA compaction was detected online using microfocused small-angle X-ray diffraction. The evolution of a DNA-dendrimer columnar square mesophase as a function of increasing dendrimer content is described. Additionally, in regions of maximum shear, an unexpected high-level perpendicular ordering of this phase is recorded. Furthermore, these assemblies are found to be in coexistence with a densely packed DNA-only mesophase in regions of excess DNA. The latter is reminiscent of dense packing found in bacteriophage and chromosomes, although surprisingly, it is not stabilized by direct dendrimer contact.

Details

show
hide
Language(s): eng - English
 Dates: 2007-06-19
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 340736
DOI: 10.1021/bm070317s
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biomacromolecules
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 8 (7) Sequence Number: - Start / End Page: 2167 - 2172 Identifier: -