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  Using microfluidic channel networks to generate gradients for studying cell migration

Rhoads, D. S., Nadkarni, S. M., Song, L., Voeltz, C., Bodenschatz, E., & Guan, J.-L. (2004). Using microfluidic channel networks to generate gradients for studying cell migration. In J.-L. Guan (Ed.), Cell Migration: Developmental Methods and Protocols (pp. 347-358). Totowa, NJ, USA: Humana Press.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-1605-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-1606-2
Genre: Book Chapter

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 Creators:
Rhoads, D. S., Author
Nadkarni, S. M., Author
Song, L., Author
Voeltz, C., Author
Bodenschatz, E.1, Author              
Guan, J.-L., Author
Affiliations:
1Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063287              

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Free keywords: gradient; microfluidics; migration; chemoattractant; microenvironment; nanobiotechnology
 Abstract: In this chapter, we will discuss a method for the generation of gradients that can be quantitatively used for studying directional cell migration. Microfluidic networks, which serially split and remix small volumes of solutions under laminar flow conditions to generate a series of microchannels of increasing protein concentration. At a juncture of these microchannels, where a single broad channel is formed, a protein concentration gradient can be easily achieved. This method is highly useful because of the ability with which we can control, manipulate and analyze chemical gradients and cells' chemotactic behavior in a quantitative manner.

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Language(s): eng - English
 Dates: 2004-11-30
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 226316
 Degree: -

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Title: Cell Migration: Developmental Methods and Protocols
Source Genre: Book
 Creator(s):
Guan, J.-L., Editor
Affiliations:
-
Publ. Info: Totowa, NJ, USA : Humana Press
Pages: - Volume / Issue: 294 Sequence Number: - Start / End Page: 347 - 358 Identifier: -