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  An unexpected reactivity of the P460 cofactor in hydroxylamine oxidoreductase

Dietl, A., Maalcke, W., & Barends, T. (2015). An unexpected reactivity of the P460 cofactor in hydroxylamine oxidoreductase. Acta Crystallographica. Section D: Biological Crystallography (Copenhagen), 71, 1708-1713. doi:10.1107/S1399004715010706.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-2883-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-2A75-9
Genre: Journal Article

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ActaCrystD_71_2015_1708.pdf (Any fulltext), 1010KB
 
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Dietl, Andreas1, Author              
Maalcke, Wouter, Author
Barends, Thomas1, Author              
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: haem ruffling; covalent modification; oxidoreductase; anaerobic ammonium oxidation
 Abstract: Hydroxylamine oxidoreductases (HAOs) contain a unique haem cofactor called P460 that consists of a profoundly ruffled c-type haem with two covalent bonds between the haem porphyrin and a conserved tyrosine. This cofactor is exceptional in that it abstracts electrons from a ligand bound to the haem iron, whereas other haems involved in redox chemistry usually inject electrons into their ligands. The effects of the tyrosine cross-links and of the haem ruffling on the chemistry of this cofactor have been investigated theoretically but are not yet clear. A new crystal structure of an HAO from Candidatus Kuenenia stuttgartiensis, a model organism for anaerobic ammonium oxidation, now shows that its P460 cofactor has yet another unexpected reactivity: when ethylene glycol was used as a cryoprotectant, the 1.8 Å resolution electron-density maps showed additional density which could be interpreted as an ethylene glycol molecule covalently bound to the C16 atom of the haem ring, opposite the covalent links to the conserved tyrosine. Possible causes for this unexpected reactivity are discussed.

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Language(s): eng - English
 Dates: 2015-02-182015-06-032015-08-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Acta Crystallographica. Section D: Biological Crystallography (Copenhagen)
  Other : Acta Crystallogr D
Source Genre: Journal
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Publ. Info: [Copenhagen, Denmark : Published for the International Union of Crystallography by Munksgaard]
Pages: - Volume / Issue: 71 Sequence Number: - Start / End Page: 1708 - 1713 Identifier: ISSN: 0907-4449
CoNE: https://pure.mpg.de/cone/journals/resource/954925562619