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  The tetraspanin web revisited by super-resolution microscopy.

Zuidscherwoude, M., Göttfert, F., Dunlock, V. M. E., Figdor, C. G., van den Bogaart, G., & van Spriel, A. B. (2015). The tetraspanin web revisited by super-resolution microscopy. Scientific Reports, 5: 12201. doi:10.1038/srep12201.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-2A3D-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0002-A556-3
Genre: Journal Article

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Zuidscherwoude, M., Author
Göttfert, F.1, Author              
Dunlock, V. M. E., Author
Figdor, C. G., Author
van den Bogaart, G., Author
van Spriel, A. B., Author
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1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: The spatial organization of membrane proteins in the plasma membrane is critical for signal transduction, cell communication and membrane trafficking. Tetraspanins organize functional higherorder protein complexes called 'tetraspanin-enriched microdomains (TEMs)' via interactions with partner molecules and other tetraspanins. Still, the nanoscale organization of TEMs in native plasma membranes has not been resolved. Here, we elucidated the size, density and distribution of TEMs in the plasma membrane of human B cells and dendritic cells using dual color stimulated emission depletion (STED) microscopy. We demonstrate that tetraspanins form individual nanoclusters smaller than 120 nm and quantified that a single tetraspanin CD53 cluster contains less than ten CD53 molecules. CD53 and CD37 domains were adjacent to and displayed only minor overlap with clusters containing tetraspanins CD81 or CD82. Moreover, CD53 and CD81 were found in closer proximity to their partners MHC class II and CD19 than to other tetraspanins. Although these results indicate that tetraspanin domains are adjacently positioned in the plasma membrane, they challenge the current view of the tetraspanin web of multiple tetraspanin species organized into a single domain. This study increases the molecular understanding of TEMs at the nanoscale level which is essential for comprehending tetraspanin function in cell biology.

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Language(s): eng - English
 Dates: 2015-07-17
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/srep12201
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Title: Scientific Reports
Source Genre: Journal
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Pages: 18 Volume / Issue: 5 Sequence Number: 12201 Start / End Page: - Identifier: -