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  Mutagenesis reveals a role for ABP/GRIP binding to GluR2 in synaptic surface accumulation of the AMPA receptor

Osten, P., Khatri, L., Perez, J. L., Köhr, G., Giese, G., Daly, C., et al. (2000). Mutagenesis reveals a role for ABP/GRIP binding to GluR2 in synaptic surface accumulation of the AMPA receptor. Neuron, 27, 313-325. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/10985351.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-2D37-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-2D38-A
Genre: Journal Article
Alternative Title : Mutagenesis reveals a role for ABP/GRIP binding to GluR2 in synaptic surface accumulation of the AMPA receptor

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 Creators:
Osten, Pavel1, Author              
Khatri, Latika, Author
Perez, Joey L., Author
Köhr, Georg1, Author              
Giese, Günter2, Author              
Daly, Christopher, Author
Schulz, Torsten Wilhelm1, Author              
Wensky, Allen, Author
Lee, Laveria M., Author
Ziff, Edward B., Author
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1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: We studied the role of PDZ proteins GRIP, ABP, and PICK1 in GluR2 AMPA receptor trafficking. An epitope-tagged MycGluR2 subunit, when expressed in hippocampal cultured neurons, was specifically targeted to the synaptic surface. With the mutant MycGluR2?1-10, which lacks the PDZ binding site, the overall dendritic intracellular transport and the synaptic surface targeting were not affected. However, over time, MycGluR2?1-10 accumulated at synapses significantly less than MycGluR2. Notably, a single residue substitution, S880A, which blocks binding to ABP/GRIP but not to PICK1, reduced synaptic accumulation to the same extent as the PDZ site truncation. We conclude that the association of GluR2 with ABP and/or GRIP but not PICK1 is essential for maintaining the synaptic surface accumulation of the receptor, possibly by limiting its endocytotic rate

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Language(s): eng - English
 Dates: 2000-02-232000-07-272000-09-202000-08-01
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Neuron
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 27 Sequence Number: - Start / End Page: 313 - 325 Identifier: ISSN: 0896-6273
CoNE: https://pure.mpg.de/cone/journals/resource/954925560565