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  Dynamic contact network between ribosomal subunits enables rapid large-scale rotation during spontaneous translocation.

Bock, L. V., Blau, C., Vaiana, A. C., & Grubmüller, H. (2015). Dynamic contact network between ribosomal subunits enables rapid large-scale rotation during spontaneous translocation. Nucleic Acids Research, 43(14), 6747-6760. doi:10.1093/nar/gkv649.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-3092-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-DC89-F
Genre: Journal Article

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Bock, L. V.1, Author              
Blau, C.1, Author              
Vaiana, A. C.1, Author              
Grubmüller, H.1, Author              
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1Department of Theoretical and Computational Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578631              

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 Abstract: During ribosomal translation, the two ribosomal subunits remain associated through intersubunit bridges, despite rapid large-scale intersubunit rotation. The absence of large barriers hindering rotation is a prerequisite for rapid rotation. Here, we investigate how such a flat free-energy landscape is achieved, in particular considering the large shifts the bridges undergo at the periphery. The dynamics and energetics of the intersubunit contact network are studied using molecular dynamics simulations of the prokaryotic ribosome in intermediate states of spontaneous translocation. Based on observed occupancies of intersubunit contacts, residues were grouped into clusters. In addition to the central contact clusters, peripheral clusters were found to maintain strong steady interactions by changing contacts in the course of rotation. The peripheral B1 bridges are stabilized by a changing contact pattern of charged residues that adapts to the rotational state. In contrast, steady strong interactions of the B4 bridge are ensured by the flexible helix H34 following the movement of protein S15. The tRNAs which span the subunits contribute to the intersubunit binding enthalpy to an almost constant degree, despite their different positions in the ribosome. These mechanisms keep the intersubunit interaction strong and steady during rotation, thereby preventing dissociation and enabling rapid rotation.

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Language(s): eng - English
 Dates: 2015-06-242015-08-18
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1093/nar/gkv649
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 43 (14) Sequence Number: - Start / End Page: 6747 - 6760 Identifier: -