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  A compendium of expression patterns of cholesterol biosynthetic enzymes in the mouse embryo.

Sisecioglu, M., Budak, H., Geffers, L., Cankaya, M., Ciftci, M., Thaller, C., et al. (2015). A compendium of expression patterns of cholesterol biosynthetic enzymes in the mouse embryo. Journal of Lipid Research, 56(8), 1551-1559. doi:10.1194/jlr.M059634.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-323A-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2296-E
Genre: Journal Article

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 Creators:
Sisecioglu, M., Author
Budak, H.1, Author              
Geffers, L.1, Author              
Cankaya, M.1, Author              
Ciftci, M., Author
Thaller, C.1, Author              
Eichele, G.1, Author              
Kufrevioglu, O. I., Author
Ozdemir, H., Author
Affiliations:
1Department of Genes and Behavior, MPI for biophysical chemistry, Max Planck Society, ou_persistent34              

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Free keywords: in situ hybridization; brain lipids; cholesterol/biosynthesis; eye; gene expression; nutrition; signal transduction; Smith-Lemli-Opitz syndrome
 Abstract: Cholesterol and its biosynthetic pathway intermediates and derivatives are required for many developmental processes including membrane biogenesis, transmembrane receptor signaling, steroid biogenesis, nuclear receptor activation, and posttranslational modification of hedgehog (Hh) proteins. To perform such multifaceted tasks depends on stringent regulation of expression of cholesterol biosynthetic enzymes (CBEs). We established for a whole organism, for the first time, the 3D expression pattern of all genes required for cholesterol biosynthesis (CBS), starting from acetyl-CoA and ending with cholesterol. This data was produced by high-throughput in situ hybridization on serial sections through the mouse fetus. The textually annotated image data were seamlessly integrated into the METscout and GenePaint public databases. This novel information helps in the understanding of why CBEs are expressed at particular locations within the fetus. For example, strong CBE expression is detected at sites of cell proliferation and also where cell growth increases membrane surface, such as in neurons sprouting axons and forming synapses. The CBE data also sheds light on the spatial relationship of cells and tissue that express sonic Hh (Shh) and produce cholesterol, respectively. We discovered that not all cells expressing Shh are capable of CBS.Jlr This finding suggests novel ways by which cholesterylation of Shh is regulated.

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Language(s): eng - English
 Dates: 2015-06-242015-08
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1194/jlr.M059634
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Title: Journal of Lipid Research
Source Genre: Journal
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Pages: - Volume / Issue: 56 (8) Sequence Number: - Start / End Page: 1551 - 1559 Identifier: -