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  Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes

Defacque, H., Egeberg, M., Habermann, A., Diakonova, M., Roy, C., Mangeat, P., et al. (2000). Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes. EMBO Journal, 19(2), 199-212. doi:10.1093/emboj/19.2.199.

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Genre: Journal Article
Alternative Title : Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes

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 Creators:
Defacque, Hélène, Author
Egeberg, Morten, Author
Habermann, Anja, Author
Diakonova, Maria, Author
Roy, Christian, Author
Mangeat, Paul, Author
Voelter, Wolfgang J., Author
Marriott, Gerard, Author
Pfannstiel, Jörg1, Author              
Faulstich, Heinz2, Author              
Griffiths, Gareth, Author
Affiliations:
1Emeritus Group Bioorganic Chemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497711              
2Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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Free keywords: actin assembly; ezrin-radixin-moesin family; latex beads; phagocytosis
 Abstract: The current study focuses on the molecular mechanisms responsible for actin assembly on a defined membrane surface: the phagosome. Mature phagosomes were surrounded by filamentous actin in vivo in two different cell types. Fluorescence microscopy was used to study in vitro actin nucleation/polymerization (assembly) on the surface of phagosomes isolated from J774 mouse macrophages. In order to prevent non-specific actin polymerization during the assay, fluorescent G-actin was mixed with thymosin beta4. The cytoplasmic side of phagosomes induced de novo assembly and barbed end growth of actin filaments. This activity varied cyclically with the maturation state of phagosomes, both in vivo and in vitro. Peripheral membrane proteins are crucial components of this actin assembly machinery, and we demonstrate a role for ezrin and/or moesin in this process. We propose that this actin assembly process facilitates phagosome/endosome aggregation prior to membrane fusion.

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Language(s): eng - English
 Dates: 1999-09-301999-07-061999-11-152000-01-172000-01-17
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 19 (2) Sequence Number: - Start / End Page: 199 - 212 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061