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  Conformational variability of recombination R-triplex formed by the mammalian telomeric sequence.

Shchyolkina, A. K., Kaluzhny, D. N., Borisova, O. F., Arndt-Jovin, D. J., Jovin, T. M., & Zhurkin, V. B. (2016). Conformational variability of recombination R-triplex formed by the mammalian telomeric sequence. Journal of Biomolecular Structure and Dynamics, 34(6), 1298-1306. doi:10.1080/07391102.2015.1077344.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-4163-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-1D52-2
Genre: Journal Article

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 Creators:
Shchyolkina, A. K., Author
Kaluzhny, D. N., Author
Borisova, O. F., Author
Arndt-Jovin, D. J.1, Author              
Jovin, T. M.1, Author              
Zhurkin, V. B., Author
Affiliations:
1Emeritus Group Laboratory of Cellular Dynamics, MPI for Biophysical Chemistry, Max Planck Society, ou_578629              

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Free keywords: R-triplex; Telomeric DNA; Thermodynamic stability; Fluorescence; Circular dichroism
 Abstract: Alignment of three nucleic acids strands, in which the third strand is identical to one of the DNA duplex strands, occurs in various cellular systems. In the case of telomeric t-loops, recognition between the DNA duplex and the homologous single strand is likely to be mediated by proteins through formation of the transient recombination-type R-triplex. Earlier, using 2-aminopurine as a fluorescent reporting base, we evaluated the thermodynamic characteristics of intramolecular R-triplex formed by a mixed nucleotide sequence. Here, we used this approach to explore a propensity of the telomeric TTAGGG repeat to form the R-triplex. The circular dichroism spectral changes detected upon formation of the R-triplex suggest that this process is accompanied by specific conformational changes in DNA, including a local destabilization of the target duplex next to a GGG run revealed by the fluorescence of the reporting 2-aminopurine base. Surprisingly, stability of the R-triplex formed by telomeric sequence depends strikingly on the counter ion, being higher for Na+ than for Li+. Taken together these findings indicate a significant conformational variability of telomeric DNA in the context of recombination-type R-triplex, a phenomenon of possible biological relevance.

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Language(s): eng - English
 Dates: 2015-10-152016
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1080/07391102.2015.1077344
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Title: Journal of Biomolecular Structure and Dynamics
Source Genre: Journal
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Pages: - Volume / Issue: 34 (6) Sequence Number: - Start / End Page: 1298 - 1306 Identifier: -