English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Amide proton transfer of carnosine in aqueous solution studied in vitro by WEX and CEST experiments.

Bodet, O., Goerke, S., Behl, N. G. R., Roeloffs, V. B., Zaiss, M., & Bachert, P. (2015). Amide proton transfer of carnosine in aqueous solution studied in vitro by WEX and CEST experiments. NMR in Biomedicine, 28(9), 1097-1103. doi:10.1002/nbm.3343.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-45C0-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-5FFB-8
Genre: Journal Article

Files

show Files
hide Files
:
2183230.pdf (Publisher version), 608KB
 
File Permalink:
-
Name:
2183230.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), MBPC; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2183230_Suppl.zip (Supplementary material), 189KB
Name:
2183230_Suppl.zip
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/zip / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Bodet, O., Author
Goerke, S., Author
Behl, N. G. R., Author
Roeloffs, V. B.1, Author              
Zaiss, M., Author
Bachert, P., Author
Affiliations:
1Biomedical NMR Research GmbH, MPI for Biophysical Chemistry, Max Planck Society, ou_578634              

Content

show
hide
Free keywords: carnosine; peptide bond; proton transfer; chemical exchange; CEST; APT; WEX; pH
 Abstract: Amide protons of peptide bonds induce an important chemical exchange saturation transfer (CEST) contrast in vivo. As a simple in vitro model for a peptide amide proton CEST effect, we suggest herein the dipeptide carnosine. We show that the metabolite carnosine creates a CEST effect and we study the properties of the exchange of the amide proton (-NH) of the carnosine peptide bond (NHCPB) in model solutions for a pH range from 6 to 8.3 and a temperature range from T = 5 degrees C to 43 degrees C by means of CEST and water exchange spectroscopy (WEX) experiments on a 3 T whole-body MR tomograph. The dependence of the NHCPB chemical exchange rate k(sw) on pH and temperature T was determined using WEX. For physiological conditions (T = 37 degrees C, pH = 7.10) we obtained k(sw) = (47.07 +/- 7.90)/s. With similar chemical shift and exchange properties to amide protons in vivo, carnosine forms a simple model system for optimization of CEST pulse sequences in vitro. The potential for direct detection of the metabolite carnosine in vivo is discussed.

Details

show
hide
Language(s): eng - English
 Dates: 2015-07-142015-09
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/nbm.3343
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: NMR in Biomedicine
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 28 (9) Sequence Number: - Start / End Page: 1097 - 1103 Identifier: -