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  Photoelectrochemical bioanalysis of guanosine monophosphate via the use of coupled enzymatic reactions at a CdS/ZnS quantum dot electrode.

Sabir, N., Khan, N., Völkner, J., Widdascheck, F., del Pino, P., Witte, G., et al. (2015). Photoelectrochemical bioanalysis of guanosine monophosphate via the use of coupled enzymatic reactions at a CdS/ZnS quantum dot electrode. Small, 11(43), 5844-5850. doi:10.1002/smll.201501883.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-465F-E Version Permalink: http://hdl.handle.net/21.11116/0000-0000-76B8-C
Genre: Journal Article

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 Creators:
Sabir, N., Author
Khan, N.1, Author              
Völkner, J., Author
Widdascheck, F., Author
del Pino, P., Author
Witte, G., Author
Riedel, M., Author
Lisdat, F., Author
Konrad, M.1, Author              
Parak, W. J., Author
Affiliations:
1Research Group of Enzyme Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578612              

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 Abstract: A photo-electrochemical sensor for the specific detection of guanosine monophosphate (GMP) is demonstrated, based on three enzymes combined in a coupled reaction assay. The first reaction involves the adenosine triphosphate (ATP)-dependent conversion of GMP to guanosine diphosphate (GDP) by guanylate kinase, which warrants substrate specificity. The reaction products ADP and GDPare co-substrates for the enzymatic conversion of phosphoenolpyruvate to pyruvate in a second reaction mediated by pyruvate kinase. Pyruvate in turn is the co-substrate for lactate dehydrogenase that generates lactate via oxidation of nicotinamide adenine dinucleotide (reduced form) NADH to NAD+. This third enzymatic reaction is electrochemically detected. For this purpose a CdS/ZnS quantum dot (QD) electrode is illuminated and the photocurrent response under fixed potential conditions is evaluated. The sequential enzyme reactions are first evaluated in solution. Subsequently, a sensor for GMP is constructed using polyelectrolytes for enzyme immobilization.

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Language(s): eng - English
 Dates: 2015-09-232015-11-18
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1002/smll.201501883
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Title: Small
Source Genre: Journal
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Pages: - Volume / Issue: 11 (43) Sequence Number: - Start / End Page: 5844 - 5850 Identifier: -