English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Ribosome binding induces repositioning of the signal recognition particle receptor on the translocon.

Kuhn, P., Draycheva, A., Vogt, A., Petriman, N. A., Sturm, L., Drepper, F., et al. (2015). Ribosome binding induces repositioning of the signal recognition particle receptor on the translocon. Journal of Cell Biology, 211(1), 91-104. doi:10.1083/jcb.201502103.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-4664-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-174F-7
Genre: Journal Article

Files

show Files
hide Files
:
2183348.pdf (Publisher version), 4MB
Name:
2183348.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2183348_Suppl.pdf (Supplementary material), 550KB
Name:
2183348_Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Kuhn, P., Author
Draycheva, A.1, Author              
Vogt, A., Author
Petriman, N. A., Author
Sturm, L., Author
Drepper, F., Author
Warscheid, B., Author
Wintermeyer, W.2, Author              
Koch, H. G., Author
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              
2Research Group of Ribosome Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578599              

Content

show
hide
Free keywords: -
 Abstract: Cotranslational protein targeting delivers proteins to the bacterial cytoplasmic membrane or to the eukaryotic endoplasmic reticulum membrane. The signal recognition particle (SRP) binds to signal sequences emerging from the ribosomal tunnel and targets the ribosome-nascent-chain complex (RNC) to the SRP receptor, termed FtsY in bacteria. FtsY interacts with the fifth cytosolic loop of SecY in the SecYEG translocon, but the functional role of the interaction is unclear. By using photo-cross-linking and fluorescence resonance energy transfer measurements, we show that FtsY–SecY complex formation is guanosine triphosphate independent but requires a phospholipid environment. Binding of an SRP–RNC complex exposing a hydrophobic transmembrane segment induces a rearrangement of the SecY–FtsY complex, which allows the subsequent contact between SecY and ribosomal protein uL23. These results suggest that direct RNC transfer to the translocon is guided by the interaction between SRP and translocon-bound FtsY in a quaternary targeting complex.

Details

show
hide
Language(s): eng - English
 Dates: 2015-10-12
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1083/jcb.201502103
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Cell Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 211 (1) Sequence Number: - Start / End Page: 91 - 104 Identifier: -