English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Energy homeostasis control in Drosophila adipokinetic hormone mutants.

Gáliková, M., Diesner, M., Klepsatel, P., Hehlert, P., Xu, Y., Bickmeyer, I., et al. (2015). Energy homeostasis control in Drosophila adipokinetic hormone mutants. Genetics, 201(2), 665-683. doi:10.1534/genetics.115.178897.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-46A4-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-0F3E-C
Genre: Journal Article

Files

show Files
hide Files
:
2183369.pdf (Publisher version), 3MB
 
File Permalink:
-
Name:
2183369.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2183369_Suppl_1.pdf (Supplementary material), 381KB
Name:
2183369_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2183369_Suppl_2.pdf (Supplementary material), 240KB
Name:
2183369_Suppl_2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2183369_Suppl_3.pdf (Supplementary material), 228KB
Name:
2183369_Suppl_3.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2183369_Suppl_4.pdf (Supplementary material), 195KB
Name:
2183369_Suppl_4.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2183369_Suppl_5.pdf (Supplementary material), 189KB
Name:
2183369_Suppl_5.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2183369_Suppl_6.pdf (Supplementary material), 194KB
Name:
2183369_Suppl_6.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2183369_Suppl_7.pdf (Supplementary material), 192KB
Name:
2183369_Suppl_7.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Gáliková, M., Author
Diesner, M., Author
Klepsatel, P., Author
Hehlert, P., Author
Xu, Y., Author
Bickmeyer, I., Author
Predel, R., Author
Kühnlein, R. P.1, Author              
Affiliations:
1Research Group of Molecular Physiology, MPI for biophysical chemistry, Max Planck Society, ou_578592              

Content

show
hide
Free keywords: -
 Abstract: Maintenance of biological functions under negative energy balance depends on mobilization of storage lipids and carbohydrates in animals. In mammals, glucagon and glucocorticoid signaling mobilizes energy reserves, whereas Adipokinetic hormones (AKHs) play a homologous role in insects. Numerous studies based in AKH injections and correlative studies in a broad range of insect species established the view that AKH acts as master regulator of energy mobilization during development, reproduction, and stress. In contrast to AKH, the second peptide, which is processed from the Akh encoded prohormone - termed Adipokinetic hormone precursor related peptide (APRP) - is functionally orphan. APRP is discussed as ecdysiotropic hormone or as scaffold peptide during AKH prohormone processing. However, as in the case of AKH, final evidence for APRP functions requires genetic mutant analysis. Here we employed CRISPR/Cas9-mediated genome engineering to create AKH and AKH plus APRP-specific mutants in the model insect Drosophila melanogaster. Lack of APRP did not affect any of the tested steroid-dependent processes. Similarly, Drosophila AKH signaling is dispensable for ontogenesis, locomotion, oogenesis, and homeostasis of lipid or carbohydrate storage until up to the end of metamorphosis. During adulthood, however, AKH regulates body fat content and the hemolymph sugar level as well as nutritional and oxidative stress responses. Finally, we provide evidence for a negative auto-regulatory loop, in Akh gene regulation.

Details

show
hide
Language(s): eng - English
 Dates: 2015-10-072015-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1534/genetics.115.178897
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Genetics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 201 (2) Sequence Number: - Start / End Page: 665 - 683 Identifier: -