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  Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography

Beitlich, T., Kühnel, K., Schulze-Briese, C., Shoeman, R. L., & Schlichting, I. (2007). Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography. Journal of Synchrotron Radiation, 14(1), 11-23. doi:10.1107/S0909049506049806.

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Alternativer Titel : Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography

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http://journals.iucr.org/s/issues/2007/01/00/xh5008/xh5008.pdf (beliebiger Volltext)
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 Urheber:
Beitlich, Thorsten1, Autor           
Kühnel, Karin1, Autor           
Schulze-Briese, Clemens, Autor
Shoeman, Robert L.1, Autor           
Schlichting, Ilme1, Autor           
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Inhalt

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Schlagwörter: radiolytic reduction; photoreduction; myoglobin; cytochrome P450; chloroperoxidase; microspectrophotometer; energy dependence; hydrated electrons; radical scavengers; radiation damage
 Zusammenfassung: The X-ray crystallographic analysis of redox-active systems may be complicated by photoreduction. Although radiolytic reduction by the probing X-ray beam may be exploited to generate otherwise short-lived reaction intermediates of metalloproteins, it is generally an undesired feature. Here, the X-ray-induced reduction of the three heme proteins myoglobin, cytochrome P450cam and chloroperoxidase has been followed by on-line UV-Vis absorption spectroscopy. All three systems showed a very rapid reduction of the heme iron. In chloroperoxidase the change of the ionization state from ferric to ferrous heme is associated with a movement of the heme-coordinating water molecule. The influence of the energy of the incident X-ray photons and of the presence of scavengers on the apparent reduction rate of ferric myoglobin crystals was analyzed.

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Sprache(n): eng - English
 Datum: 2006-06-122006-11-212006-12-152007-01-01
 Publikationsstatus: Erschienen
 Seiten: 13
 Ort, Verlag, Ausgabe: -
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 Art der Begutachtung: Expertenbegutachtung
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Titel: Journal of Synchrotron Radiation
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Copenhagen, Denmark : Published for the International Union of Crystallography by Munksgaard
Seiten: - Band / Heft: 14 (1) Artikelnummer: - Start- / Endseite: 11 - 23 Identifikator: ISSN: 0909-0495
CoNE: https://pure.mpg.de/cone/journals/resource/954925562624