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  Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography

Beitlich, T., Kühnel, K., Schulze-Briese, C., Shoeman, R. L., & Schlichting, I. (2007). Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography. Journal of Synchrotron Radiation, 14(1), 11-23. doi:10.1107/S0909049506049806.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-479F-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-47A0-E
Genre: Journal Article
Alternative Title : Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography

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JSynchrotronRad_14_2007_11.pdf (Any fulltext), 2MB
 
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 Creators:
Beitlich, Thorsten1, Author              
Kühnel, Karin1, Author              
Schulze-Briese, Clemens, Author
Shoeman, Robert L.1, Author              
Schlichting, Ilme1, Author              
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: radiolytic reduction; photoreduction; myoglobin; cytochrome P450; chloroperoxidase; microspectrophotometer; energy dependence; hydrated electrons; radical scavengers; radiation damage
 Abstract: The X-ray crystallographic analysis of redox-active systems may be complicated by photoreduction. Although radiolytic reduction by the probing X-ray beam may be exploited to generate otherwise short-lived reaction intermediates of metalloproteins, it is generally an undesired feature. Here, the X-ray-induced reduction of the three heme proteins myoglobin, cytochrome P450cam and chloroperoxidase has been followed by on-line UV-Vis absorption spectroscopy. All three systems showed a very rapid reduction of the heme iron. In chloroperoxidase the change of the ionization state from ferric to ferrous heme is associated with a movement of the heme-coordinating water molecule. The influence of the energy of the incident X-ray photons and of the presence of scavengers on the apparent reduction rate of ferric myoglobin crystals was analyzed.

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Language(s): eng - English
 Dates: 2006-06-122006-11-212006-12-152007-01-01
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Journal of Synchrotron Radiation
Source Genre: Journal
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Publ. Info: Copenhagen, Denmark : Published for the International Union of Crystallography by Munksgaard
Pages: - Volume / Issue: 14 (1) Sequence Number: - Start / End Page: 11 - 23 Identifier: ISSN: 0909-0495
CoNE: https://pure.mpg.de/cone/journals/resource/954925562624