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  Synthesis and characterization of allosteric probes of substrate channeling in the tryptophan synthase bienzyme complex

Ngo, H., Harris, R., Kimmich, N., Casino, P., Niks, D., Blumenstein, L., et al. (2007). Synthesis and characterization of allosteric probes of substrate channeling in the tryptophan synthase bienzyme complex. Biochemistry, 46(26), 7713-7727. doi:10.1021/bi700385f.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-494A-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-494B-F
Genre: Journal Article
Alternative Title : Synthesis and characterization of allosteric probes of substrate channeling in the tryptophan synthase bienzyme complex

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 Creators:
Ngo, Huu, Author
Harris, Rodney, Author
Kimmich, Novelle, Author
Casino, Patricia, Author
Niks, Dimitri, Author
Blumenstein, Lars1, Author              
Barends, Thomas1, Author              
Kulik, Victor1, Author              
Weyand, Michael, Author
Schlichting, Ilme1, Author              
Dunn, Michael F., Author
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Allosteric interactions regulate substrate channeling in Salmonella typhimurium tryptophan synthase. The channeling of indole between the alpha- and beta-sites via the interconnecting 25 A tunnel is regulated by allosteric signaling arising from binding of ligand to the alpha-site, and covalent reaction of l-Ser at the beta-site. This signaling switches the alpha- and beta-subunits between open conformations of low activity and closed conformations of high activity. Our objective is to synthesize and characterize new classes of alpha-site ligands (ASLs) that mimic the binding of substrates, 3-indole-d-glycerol 3'-phosphate (IGP) or d-glyceraldehyde 3-phosphate (G3P), for use in the investigation of alpha-site-beta-site interactions. The new synthesized IGP analogues contain an aryl group linked to an O-phosphoethanolamine moiety through amide, sulfonamide, or thiourea groups. The G3P analogue, thiophosphoglycolohydroxamate, contains a hydroxamic acid group linked to a thiophosphate moiety. Crystal structures of the internal aldimine complexed with G3P and with three of the new ASLs are presented. These structural and solution studies of the ASL complexes with the internal aldimine form of the enzyme establish the following. (1) ASL binding occurs with high specificity and relatively high affinities at the alpha-site. (2) Binding of the new ASLs slows the entry of indole analogues into the beta-site by blocking the tunnel opening at the alpha-site. (3) ASL binding stabilizes the closed conformations of the beta-subunit for the alpha-aminoacrylate and quinonoid forms of the enzyme. (4) The new ASLs exhibit allosteric properties that parallel the behaviors of IGP and G3P.

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Language(s): eng - English
 Dates: 2007-04-172007-02-252007-06-092007-06-092007-07-03
 Publication Status: Published in print
 Pages: 15
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 Table of Contents: -
 Rev. Type: Peer
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Title: Biochemistry
Source Genre: Journal
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Publ. Info: Columbus, Ohio : American Chemical Society
Pages: - Volume / Issue: 46 (26) Sequence Number: - Start / End Page: 7713 - 7727 Identifier: ISSN: 0006-2960
CoNE: https://pure.mpg.de/cone/journals/resource/954925384103