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  Ether à go-go potassium channel expression in soft tissue sarcoma patients

Mello de Queiroz, F., Suarez-Kurtz, G., Stühmer, W., & Pardo, L. A. (2006). Ether à go-go potassium channel expression in soft tissue sarcoma patients. Molecular Cancer, 5: 42. doi:10.1186/1476-4598-5-42.

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 Urheber:
Mello de Queiroz, Fernanda1, Autor           
Suarez-Kurtz, Guilherme, Autor
Stühmer, Walter1, Autor           
Pardo, Luis A.1, Autor           
Affiliations:
1Molecular biology of neuronal signals, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173656              

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Schlagwörter: EAG K+ CHANNELS; MELANOMA-CELLS; PROLIFERATION; IMIPRAMINE
 Zusammenfassung: Background: The expression of the human Eag1 potassium channel (Kv10.1) is normally restricted to the adult brain, but it has been detected in both tumour cell lines and primary tumours. Our purpose was to determine the frequency of expression of Eag1 in soft tissue sarcoma and its potential clinical implications. Results: We used specific monoclonal antibodies to determine the expression levels of Eag1 in soft tissue sarcomas from 210 patients by immunohistochemistry. Eag1 was expressed in 71% of all tumours, with frequencies ranging from 56% (liposarcoma) to 82% (rhabdomyosarcoma). We detected differences in expression levels depending on the histological type, but no association was seen between expression of this protein and sex, age, grade or tumour size. Four cell lines derived from relevant sarcoma histological types (fibrosarcoma and rhabdomyosarcoma) were tested for Eag1 expression by real-time RT-PCR. We found all four lines to be positive for Eag1. In these cell lines, blockage of Eag1 by RNA interference led to a decrease in proliferation. Conclusion: Eag1 is aberrantly expressed in over 70% sarcomas. In sarcoma cell lines, inhibition of Eag1 expression and/or function leads to reduced proliferation. The high frequency of expression of Eag1 in primary tumours and the restriction of normal expression of the channel to the brain, suggests the application of this protein for diagnostic or therapeutic purposes.

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Sprache(n): eng - English
 Datum: 2006-10
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 292149
ISI: 000241387400001
ISI: 000241387400001
DOI: 10.1186/1476-4598-5-42
 Art des Abschluß: -

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Titel: Molecular Cancer
  Alternativer Titel : Mol. Cancer
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 5 Artikelnummer: 42 Start- / Endseite: - Identifikator: ISSN: 1476-4598