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  Silencing the activity and proliferative properties of the human EagI potassium channel by RNA interference

Weber, C., Mello de Queiroz, F., Downie, B. R., Suckow, A., Stühmer, W., & Pardo, L. A. (2006). Silencing the activity and proliferative properties of the human EagI potassium channel by RNA interference. Journal of Biological Chemistry, 281(19), 13030-13037.

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 Creators:
Weber, Claudia1, Author              
Mello de Queiroz, Fernanda1, Author              
Downie, Bryan R.1, Author              
Suckow, Arnt1, Author              
Stühmer, Walter1, Author              
Pardo, Luis A.1, Author              
Affiliations:
1Molecular biology of neuronal signals, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173656              

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Free keywords: DOUBLE-STRANDED-RNA; K+ CHANNELS; MAMMALIAN-CELLS; GENE-EXPRESSION; ACTIVATION; DROSOPHILA; ETHER; BLOCK; INHIBITION; ASTEMIZOLE
 Abstract: EagI potassium channels are natively expressed in the mammalian brain as well as in many cancer cell lines and tumor tissues. The role of EagI in malignant transformation has been suggested by several experiments, but the lack of specific EagI inhibitors has made it difficult to examine the influence of the channel on oncogenesis and its potential as a therapeutic target. We have used short interfering RNA to test the effects of EagI reduction on the behavior of tumor cells in vitro. By generating and optimizing an EagI-specific short interfering RNA system, we were able to study the effects of EagI depletion on several cancer cell lines that endogenously express this protein. We show here that our short interfering RNA sequences act specifically on EagI, reproducibly induce a significant decrease in the proliferation of tumor cell lines, and do not trigger any observable nonspecific responses.

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Language(s): eng - English
 Dates: 2006-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 292190
ISI: 000237336600006
ISI: 000237336600006
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Title: Journal of Biological Chemistry
  Alternative Title : J. Biol. Chem.
Source Genre: Journal
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Pages: - Volume / Issue: 281 (19) Sequence Number: - Start / End Page: 13030 - 13037 Identifier: ISSN: 0021-9258