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  Identification and characterization of a stress-inducible and a constitutive small heat-shock protein targeted to the matrix of plant peroxisomes

Ma, C., Haslbeck, M., Babujee, L., Jahn, O., & Reumann, S. (2006). Identification and characterization of a stress-inducible and a constitutive small heat-shock protein targeted to the matrix of plant peroxisomes. Plant Physiology, 141(1), 47-60.

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 Urheber:
Ma, Changle, Autor
Haslbeck, Martin, Autor
Babujee, Lavanya, Autor
Jahn, Olaf1, Autor           
Reumann, Sigrun, Autor
Affiliations:
1Proteomics, Wiss. Servicegruppen, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173673              

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Schlagwörter: ARABIDOPSIS-THALIANA; ALPHA-CRYSTALLIN; MOLECULAR CHAPERONES; SACCHAROMYCES-CEREVISIAE; MALATE-DEHYDROGENASE; PROTEOMIC ANALYSIS; LEAF PEROXISOMES; OXIDATIVE STRESS; IN-VIVO; COTYLEDONS
 Zusammenfassung: Small heat-shock proteins (sHsps) are widespread molecular chaperones for which a peroxisomal localization has not yet been reported. The Arabidopsis (Arabidopsis thaliana) genome encodes two sHsps with putative peroxisomal targeting signals type 1 or 2 (PTS1 or PTS2). As demonstrated by double-labeling experiments using full-length fusion proteins with enhanced yellow fluorescent protein and deletion constructs lacking the putative targeting domains, AtHsp15.7 (At5g37670) and AtAcd31.2 (At1g06460) are targeted to the peroxisome matrix by a functional PTS1 (SKL >) and a functional PTS2 (RLx(5)HF), respectively. The peroxisomal localization of AtAcd31.2 was further confirmed by isolation of leaf peroxisomes from Arabidopsis by two successive sucrose density gradients, protein separation by one- and two-dimensional gel electrophoresis, and mass spectrometric protein identification. When AtHsp15.7 and AtAcd31.2 were heterologously expressed in yeast ( Saccharomyces cerevisiae) and directed to the cytosol by deletion of the PTSs, both sHsps were able to complement the morphological phenotype of yeast mutants deficient in the cytosolic homologs ScHsp42 or ScHsp26. According to expression studies by reverse transcription-PCR, AtAcd31.2 is constitutively expressed, whereas AtHsp15.7 is hardly expressed under normal conditions but strongly induced by heat and oxidative stress, the latter of which was triggered by the catalase inhibitor 3-aminotriazole or the herbicide methyl viologen applied by watering of whole plants or infiltration of rosette leaves. Thus, plants are exceptional among eukaryotes in employing sHsps in the peroxisome matrix to prevent unspecific aggregation of partially denatured proteins under both physiological and stress conditions.

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Sprache(n): eng - English
 Datum: 2006-05
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 299327
ISI: 000237409400005
ISI: 000237409400005
 Art des Abschluß: -

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Titel: Plant Physiology
  Alternativer Titel : Plant Physiology
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 141 (1) Artikelnummer: - Start- / Endseite: 47 - 60 Identifikator: ISSN: 0032-0889