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  Segregated expression of AMPA-type glutamate receptors and glutamate transporters defines distinct astrocyte populations in the mouse hippocampus

Matthias, K., Kirchhoff, F., Seifert, G., Hüttmann, K., Matyash, M., Kettenmann, H., et al. (2003). Segregated expression of AMPA-type glutamate receptors and glutamate transporters defines distinct astrocyte populations in the mouse hippocampus. The Journal of Neuroscience: the Official Journal of the Society for Neuroscience, 23(5), 1750-1758.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-2728-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-2729-D
Genre: Journal Article

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 Creators:
Matthias, Katja, Author
Kirchhoff, Frank1, Author              
Seifert, Gerald, Author
Hüttmann, Kerstin, Author
Matyash, Marina, Author
Kettenmann, Helmut, Author
Steinhäuser, Christian, Author
Affiliations:
1Neurogenetics, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173664              

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Free keywords: glial cells; astrocytes; astron; AMPA receptors; glutamate transporters; patch clamp; hippocampus; GFAP promoter- controlled EGFP expression; transgenic mouse; NG2; S100b
 Abstract: Recent data have suggested the existence of direct signaling pathways between glial cells and neurons. Here we report the coexistence of distinct types of cells expressing astrocyte- specific markers within the hippocampus that display diverse morphological, molecular, and functional profiles. Usage of transgenic mice with GFAP promoter-controlled enhanced green fluorescent protein (EGFP) expression allowed the identification of astroglial cells after fresh isolation or in brain slices. Combining patch-clamp recordings and single-cell reverse transcription-PCR, we distinguished two morphologically distinct types of EGFP-positive cells, one expressing glutamate transporters and the other expressing ionotropic glutamate receptors. None of the EGFP-positive cells coexpressed glutamate receptors and transporters. Subpopulations of glutamate receptor-bearing EGFP-positive cells expressed AN2, the mouse homolog of the rat NG2 proteoglycan or transcripts for excitatory amino acid carrier 1, a neuronal glutamate transporter. Our data demonstrate the presence of distinct, independent populations of cells with astroglial properties in the developing hippocampus that can differently modulate neuronal signaling pathways. The observed heterogeneity of cells with GFAP promoter-regulated EGFP expression and S100beta/GFAP immunoreactivity challenges the hitherto accepted definition of astrocytes.

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Language(s): eng - English
 Dates: 2003-03-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 42793
ISI: 000181450600024
 Degree: -

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Title: The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
  Other : J. Neurosci.
Source Genre: Journal
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Publ. Info: Baltimore, MD : The Society
Pages: - Volume / Issue: 23 (5) Sequence Number: - Start / End Page: 1750 - 1758 Identifier: ISSN: 0270-6474
CoNE: /journals/resource/954925502187_1