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  Multiparameter evanescent-wave imaging in biological fluorescence microscopy

Oheim, M., & Stühmer, W. (2002). Multiparameter evanescent-wave imaging in biological fluorescence microscopy. IEEE Journal of Quantum Electronics, 38(2), 142-148.

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 Creators:
Oheim, M., Author
Stühmer, Walter1, Author           
Affiliations:
1Molecular biology of neuronal signals, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173656              

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Free keywords: fluorescence; fluorescence quenching; polarization; TIRF; variable angle
 Abstract: Wide-field optical microscopy of live cells with near-field precision, tracking the dynamics of subcellular organelles, imaging of single-molecule reactions with millisecond time- resolution, or watching synaptic nerve terminals "in action"- these are some examples of recent work that triggered the "renaissance" of evanescent-wave (EW) spectroscopy in biological imaging. In these studies, the goal is to markedly reduce background fluorescence from locations in the sample other than the cell/substrate interface. After a brief reminder of EW generation, we discuss how the confinement of fluorescence excitation highlights cellular structure near the plasma membrane with unprecedented detail. We then illustrate how the intensity distribution and polarization of the EW can be used to study dynamic biological processes that have neither been accessible with optical (confocal or two-photon fluorescence) nor electron microscopy, and take a glimpse of what is to come in EW imaging.

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Language(s): eng - English
 Dates: 2002-02
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 17456
ISI: 000173483000005
 Degree: -

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Title: IEEE Journal of Quantum Electronics
  Other : IEEE J. Quantum Electron.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: [New York : Institute of Electrical and Electronics Engineers
Pages: - Volume / Issue: 38 (2) Sequence Number: - Start / End Page: 142 - 148 Identifier: ISSN: 0018-9197
CoNE: https://pure.mpg.de/cone/journals/resource/954925405667