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  Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography

Mueller, C., Marx, A., Epp, S. W., Zhong, Y. P., Kuo, A., Balo, A. R., et al. (2015). Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography. Structural Dynamics, 2(5): 054302. doi:10.1063/1.4928706.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-4E67-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-4E68-3
Genre: Journal Article

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http://dx.doi.org/10.1063/1.4928706 (Publisher version)
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 Creators:
Mueller, C.1, Author
Marx, Alexander2, Author              
Epp, Sascha W.2, Author              
Zhong, Yin Peng2, Author              
Kuo, A.3, Author
Balo, A. R.3, Author
Soman, J.4, Author
Schotte, F.5, Author
Lemke, H. T.6, Author
Owen, R. L.7, Author
Pai, E. F.3, 8, 9, Author
Pearson, A. R.10, Author
Olson, J. S.4, Author
Anfinrud, P. A.5, Author
Ernst, O. P.3, 9, Author
Miller, R. J. Dwayne1, 2, 10, Author              
Affiliations:
1Departments of Chemistry and Physics, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada, ou_persistent22              
2Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
3Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada, ou_persistent22              
4Department of BioSciences, Rice University, Houston, Texas 77251-1892, USA, ou_persistent22              
5Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA, ou_persistent22              
6LCLS, SLAC National Accelerator Laboratory, Menlo Park, California 94025, USA, ou_persistent22              
7Diamond Light Source, Harwell Campus for Science and Innovation, Didcot OX11 0DE, United Kingdom, ou_persistent22              
8Department of Medical Biophysics, University of Toronto and Campbell Family Institute for Cancer Research, University Health Network, Toronto, Ontario M5G 1L7, Canada, ou_persistent22              
9Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada, ou_persistent22              
10Hamburg Centre for Ultrafast Imaging, University of Hamburg, CFEL, Building 99, Luruper Chaussee 149, 22761 Hamburg, Germany, ou_persistent22              

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Free keywords: X-ray diffraction; Crystal structure; Proteins; Crystallization; Time dependent material structure
 Abstract: We present a crystallography chip enabling in situ room temperature crystallography at microfocus synchrotron beamlines and X-ray free-electron laser (X-FEL) sources. Compared to other in situ approaches, we observe extremely low background and high diffraction data quality. The chip design is robust and allows fast and efficient loading of thousands of small crystals. The ability to load a large number of protein crystals, at room temperature and with high efficiency, into prescribed positions enables high throughput automated serial crystallography with microfocus synchrotron beamlines. In addition, we demonstrate the application of this chip for femtosecond time-resolved serial crystallography at the Linac Coherent Light Source (LCLS, Menlo Park, California, USA). The chip concept enables multiple images to be acquired from each crystal, allowing differential detection of changes in diffraction intensities in order to obtain high signal-to-noise and fully exploit the time resolution capabilities of XFELs.

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Language(s): eng - English
 Dates: 2015-04-212015-08-062015-08-18
 Publication Status: Published online
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1063/1.4928706
 Degree: -

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Title: Structural Dynamics
Source Genre: Journal
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Publ. Info: Melville, NY : American Institute of Physics
Pages: - Volume / Issue: 2 (5) Sequence Number: 054302 Start / End Page: - Identifier: Other: 2329-7778
CoNE: /journals/resource/2329-7778