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  Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix

Cocheme, H. M., Quin, C., McQuaker, S. J., Cabreiro, F., Logan, A., Prime, T. A., et al. (2011). Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix. Cell Metab, 13(3), 340-50. doi:S1550-4131(11)00047-7 [pii] 10.1016/j.cmet.2011.02.003.

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 Creators:
Cocheme, H. M.1, Author
Quin, C.1, Author
McQuaker, S. J.1, Author
Cabreiro, F.1, Author
Logan, A.1, Author
Prime, T. A.1, Author
Abakumova, I.1, Author
Patel, J. V.1, Author
Fearnley, I. M.1, Author
James, A. M.1, Author
Porteous, C. M.1, Author
Smith, R. A.1, Author
Saeed, S.1, Author
Carre, J. E.1, Author
Singer, M.1, Author
Gems, D.1, Author
Hartley, R. C.1, Author
Partridge, L.1, Author
Murphy, M. P.1, Author
Affiliations:
1Max Planck Society, ou_persistent13              

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Free keywords: Aging Animals Chromatography, High Pressure Liquid/*methods Drosophila/metabolism Hydrogen Peroxide/*analysis Mitochondria/*metabolism Organophosphorus Compounds/*analysis/chemistry Phenols/*analysis/chemistry Tandem Mass Spectrometry/*methods
 Abstract: Hydrogen peroxide (H(2)O(2)) is central to mitochondrial oxidative damage and redox signaling, but its roles are poorly understood due to the difficulty of measuring mitochondrial H(2)O(2) in vivo. Here we report a ratiometric mass spectrometry probe approach to assess mitochondrial matrix H(2)O(2) levels in vivo. The probe, MitoB, comprises a triphenylphosphonium (TPP) cation driving its accumulation within mitochondria, conjugated to an arylboronic acid that reacts with H(2)O(2) to form a phenol, MitoP. Quantifying the MitoP/MitoB ratio by liquid chromatography-tandem mass spectrometry enabled measurement of a weighted average of mitochondrial H(2)O(2) that predominantly reports on thoracic muscle mitochondria within living flies. There was an increase in mitochondrial H(2)O(2) with age in flies, which was not coordinately altered by interventions that modulated life span. Our findings provide approaches to investigate mitochondrial ROS in vivo and suggest that while an increase in overall mitochondrial H(2)O(2) correlates with aging, it may not be causative.

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Language(s): eng - English
 Dates: 2011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: 21356523
DOI: S1550-4131(11)00047-7 [pii] 10.1016/j.cmet.2011.02.003
ISSN: 1932-7420 (Electronic) 1550-4131 (Linking)
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Title: Cell Metab
Source Genre: Journal
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Pages: - Volume / Issue: 13 (3) Sequence Number: - Start / End Page: 340 - 50 Identifier: -