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  Use of ddPCR in experimental evolution studies

Koch, H., Jeschke, A., & Becks, L. (2015). Use of ddPCR in experimental evolution studies. Methods in Ecology and Evolution. doi:10.1111/2041-210X.12467.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-538D-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-538E-C
Genre: Journal Article

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Koch_et_al_2015.pdf (Preprint), 879KB
 
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 Creators:
Koch, Hanna1, Author              
Jeschke, Alina, Author
Becks, Lutz1, Author              
Affiliations:
1Research Group Community Dynamics, Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445642              

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Free keywords: ddPCR; experimental evolution; mating types; genotypes; multiplex; Chlamydomonas; microbes; allele frequencies; digital PCR
 Abstract: 1. Experimental evolution is an important research framework for evolutionary biologists as it allows direct testing of fundamental theories about adaptation and diversity, which often requires the tracking of genotypes or alleles over time. This however requires tools such as genetic markers, distinguishable morphological characters or genotyping, which can be time and labor intensive, and especially if high-throughput processing is necessary. 2. Here we present a novel approach of combining multiplex ddPCR with experimental evolution for tracking genotype frequencies in different experimental populations over time. 3. We found this method to be both precise and accurate for detecting and quantifying targets of interest, especially as compared to traditional PCR methods. 4. The use of multiplex ddPCR to follow frequencies can be used for a wide range of experimental evolution applications.

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Language(s): eng - English
 Dates: 2015-08-082015-07-032015-08-102015-08-29
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1111/2041-210X.12467
 Degree: -

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Title: Methods in Ecology and Evolution
Source Genre: Journal
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Publ. Info: London, UK : John Wiley and Sons Inc.
Pages: 30 S Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: Other: 2041-210X
CoNE: /journals/resource/2041-210X