English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Single-molecule modeling of mRNA degradation by miRNA: Lessons from data

Sin, C., Chiarugi, D., & Valleriani, A. (2015). Single-molecule modeling of mRNA degradation by miRNA: Lessons from data. BMC Systems Biology, 9(Suppl 3): S2. doi:10.1186/1752-0509-9-S3-S2.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-5666-A Version Permalink: http://hdl.handle.net/21.11116/0000-0002-798F-6
Genre: Journal Article

Files

show Files
hide Files
:
2191456.pdf (Publisher version), 3MB
Name:
2191456.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
2191456_supp.pdf (Supplementary material), 97KB
Name:
2191456_supp.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Sin, Celine1, Author              
Chiarugi, Davide1, Author              
Valleriani, Angelo1, Author              
Affiliations:
1Angelo Valleriani, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863324              

Content

show
hide
Free keywords: Open Access; miRNA; mRNA complex degradation; mRNA decay; stochastic modeling; single molecule; open access
 Abstract: Recent experimental results on the effect of miRNA on the decay of its target mRNA have been analyzed against a previously hypothesized single molecule degradation pathway. According to that hypothesis, the silencing complex (miRISC) first interacts with its target mRNA and then recruits the protein complexes associated with NOT1 and PAN3 to trigger deadenylation (and subsequent degradation) of the target mRNA. Our analysis of the experimental decay patterns allowed us to refine the structure of the degradation pathways at the single molecule level. Surprisingly, we found that if the previously hypothesized network was correct, only about 7% of the target mRNA would be regulated by the miRNA mechanism, which is inconsistent with the available knowledge. Based on systematic data analysis, we propose the alternative hypothesis that NOT1 interacts with miRISC before binding to the target mRNA. Moreover, we show that when miRISC binds alone to the target mRNA, the mRNA is degraded more slowly, probably through a deadenylation-independent pathway. The new biochemical pathway proposed here both fits the data and paves the way for new experimental work to identify new interactions.

Details

show
hide
Language(s):
 Dates: 2015-06-012015
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1186/1752-0509-9-S3-S2
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: BMC Systems Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 9 (Suppl 3) Sequence Number: S2 Start / End Page: - Identifier: ISSN: 1752-0509