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  An atypical CNG channel activated by a single cGMP molecule controls sperm chemotaxis

Bönigk, W., Loogen, A., Seifert, R., Kashikar, N., Klemm, C., Krause, E., et al. (2009). An atypical CNG channel activated by a single cGMP molecule controls sperm chemotaxis. Science Signaling, 2(ra68).

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Bönigk, W.1, Author           
Loogen, A.1, Author           
Seifert, R.1, Author           
Kashikar, N.1, Author           
Klemm, C., Author
Krause, E., Author
Hagen, V., Author
Kremmer, E., Author
Strünker, T.1, Author           
Kaupp, U. B.1, Author           
Affiliations:
1Department of Molecular Sensory Systems, Center of Advanced European Studies and Research (caesar), Max Planck Society, ou_2173679              

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Free keywords: CNG
 Abstract: Sperm of the sea urchin Arbacia punctulata can respond to a single molecule of chemoattractant released by an egg. The mechanism underlying this extreme sensitivity is unknown. Crucial signaling events in the response of A. punctulata sperm to chemoattractant include the rapid synthesis of the intracellular messenger guanosine 3',5'-monophosphate (cGMP) and the ensuing membrane hyperpolarization that results from the opening of potassium-selective cyclic nucleotide-gated (CNGK) channels. Here, we use calibrated photolysis of caged cGMP to show that similar to 45 cGMP molecules are generated during the response to a single molecule of chemoattractant. The CNGK channel can respond to such small cGMP changes because it is exquisitely sensitive to cGMP and activated in a noncooperative fashion. Like voltage-activated Cav and Nav channels, the CNGK polypeptide consists of four homologous repeat sequences. Disabling each of the four cyclic nucleotide-binding sites through mutagenesis revealed that binding of a single cGMP molecule to repeat 3 is necessary and sufficient to activate the CNGK channel. Thus, CNGK has developed a mechanism of activation that is different from the activation of other CNG channels, which requires the cooperative binding of several ligands and operates in the micromolar rather than the nanomolar range

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 Dates: 2009
 Publication Status: Issued
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 Identifiers: ISI: ISI:000275644000006
ISSN: 1937-9145
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Title: Science Signaling
  Alternative Title : Sci. Signal.
Source Genre: Journal
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Pages: - Volume / Issue: 2 (ra68) Sequence Number: - Start / End Page: - Identifier: -