English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Pseudo-complementary PNA actuators as reversible switches in dynamic DNA nanotechnology

Ackermann, D., & Famulok, M. (2013). Pseudo-complementary PNA actuators as reversible switches in dynamic DNA nanotechnology. Nucleic acids research, 41(8), 4729-39. doi:10.1093/nar/gkt121.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-63FC-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-63FD-2
Genre: Journal Article

Files

show Files
hide Files
:
Ackermann-2013-Pseudo-complementary.pdf (Any fulltext), 5MB
 
File Permalink:
-
Name:
Ackermann-2013-Pseudo-complementary.pdf
Description:
-
Visibility:
Private
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Description:
-

Creators

show
hide
 Creators:
Ackermann, D., Author
Famulok, M.1, Author
Affiliations:
1External Organizations, ou_persistent22              

Content

show
hide
Free keywords: DNA/chemical synthesis/*chemistry Nanostructures/*chemistry Peptide Nucleic Acids/chemical synthesis/*chemistry Rotaxanes/chemistry
 Abstract: The structural reorganization of nanoscale DNA architectures is a fundamental aspect in dynamic DNA nanotechnology. Commonly, DNA nanoarchitectures are reorganized by means of toehold-expanded DNA sequences in a strand exchange process. Here we describe an unprecedented, toehold-free switching process that relies on pseudo-complementary peptide nucleic acid (pcPNA) by using a mechanism that involves double-strand invasion. The usefulness of this approach is demonstrated by application of these peptide nucleic acids (PNAs) as switches in a DNA rotaxane architecture. The monomers required for generating the pcPNA were obtained by an improved synthesis strategy and were incorporated into a PNA actuator sequence as well as into a short DNA strand that subsequently was integrated into the rotaxane architecture. Alternate addition of a DNA and PNA actuator sequence allowed the multiple reversible switching between a mobile rotaxane macrocycle and a stationary pseudorotaxane state. The switching occurs in an isothermal process at room temperature and is nearly quantitative in each switching step. pcPNAs can potentially be combined with light- and toehold-based switches, thus broadening the toolbox of orthogonal switching approaches for DNA architectures that open up new avenues in dynamic DNA nanotechnology.

Details

show
hide
Language(s):
 Dates: 2013
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: 23444144
DOI: 10.1093/nar/gkt121
ISSN: 1362-4962 (Electronic)
ISSN: 0305-1048 (Linking)
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nucleic acids research
  Alternative Title : Nucleic Acids Res.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 41 (8) Sequence Number: - Start / End Page: 4729 - 39 Identifier: -