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  A luminescent oxygen channeling biosensor that measures small GTPase activation

Niebel, B., Weiche, B., Mueller, A. L., Li, D. Y., Karnowski, N., & Famulok, M. (2011). A luminescent oxygen channeling biosensor that measures small GTPase activation. Chemical Communications, 47(26), 7521-3. doi:10.1039/c1cc11944c.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0028-6423-2 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0028-6424-F
Genre: Journal Article

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Niebel-2011-A luminescent oxygen channeling bi.pdf (Any fulltext), 720KB
 
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http://www.ncbi.nlm.nih.gov/pubmed/21625685 (Any fulltext)
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http://pubs.rsc.org/en/Content/ArticleLanding/2011/CC/c1cc11944c (Any fulltext)
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 Creators:
Niebel, B., Author
Weiche, B., Author
Mueller, A. L., Author
Li, D. Y., Author
Karnowski, N., Author
Famulok, M.1, Author
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1External Organizations, ou_persistent22              

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Free keywords: Animals Biosensing Techniques/*methods Enzyme Activation *Luminescent Measurements Mice Monomeric GTP-Binding Proteins/*metabolism NIH 3T3 Cells Oxygen/*metabolism Time Factors
 Abstract: We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining activation states of diverse GTPases in various biological contexts.

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 Dates: 2011
 Publication Status: Issued
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 Identifiers: Other: 21625685
DOI: 10.1039/c1cc11944c
ISSN: 1364-548X (Electronic)
ISSN: 1359-7345 (Linking)
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Title: Chemical Communications
  Alternative Title : Chem. Commun. (Camb)
Source Genre: Journal
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Pages: - Volume / Issue: 47 (26) Sequence Number: - Start / End Page: 7521 - 3 Identifier: -