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  Tandem affinity purification combined with mass spectrometry to identify components of protein complexes

Kaiser, P., Meierhofer, D., Wang, X., & Huang, L. (2008). Tandem affinity purification combined with mass spectrometry to identify components of protein complexes. In M. Starkey, & R. Elaswarapu (Eds.), Methods in molecular biology (2nd edition, pp. 309-326). Humana Press.

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Kaiser.pdf (Publisher version), 239KB
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© 2008 Springer International Publishing AG, Part of Springer Science+Business Media
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 Creators:
Kaiser, Peter1, Author
Meierhofer, David1, 2, Author              
Wang, Xiaorong , Author
Huang, Lan, Author
Affiliations:
1Department of Biological Chemistry, College of Medicine, University of California Irvine, Irvine, CA, USA , ou_persistent22              
2Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479669              

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Free keywords: tandem-affinity purification (TAP), His-Bio tag, mass spectrometry, protein complex identification, in-vivo cross-linking, MudPIT
 Abstract: Most biological processes are governed by multiprotein complexes rather than individual proteins. Identification of protein complexes therefore is becoming increasingly important to gain a molecular understanding of cells and organisms. Mass spectrometry—based proteomics combined with affinity-tag-based protein purification is one of the most effective strategies to isolate and identify protein complexes. The development of tandem-affinity purification approaches has revolutionized proteomics experiments. These two-step affinity purification strategies allow rapid, effective purification of protein complexes and, at the same time, minimize background. Identification of even very low-abundant protein complexes with modern sensitive mass spectrometers has become routine. Here, we describe two general strategies for tandem-affinity purification followed by mass spectrometric identification of protein complexes.

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Language(s): eng - English
 Dates: 2008
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-59745-188-8_21
 Degree: -

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Title: Methods in molecular biology
  Subtitle : Genomics Protocols
Source Genre: Series
 Creator(s):
Starkey, Mike, Editor
Elaswarapu, Ramnath, Editor
Affiliations:
-
Publ. Info: Humana Press, 2nd edition
Pages: 425 Volume / Issue: 439 Sequence Number: - Start / End Page: 309 - 326 Identifier: ISBN: 978-1-58829-871-3