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  Global Analysis of Ubiquitination

Meierhofer, D., & Kaiser, P. (2011). Global Analysis of Ubiquitination. In K. W. Li (Ed.), Neuroproteomics.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-8FB3-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-8FC0-6
Genre: Book Chapter

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Meierhofer.pdf (Publisher version), 279KB
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 Creators:
Meierhofer, David1, Author              
Kaiser, Peter, Author
Affiliations:
1Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479669              

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Free keywords: Ubiquitination, SILAC, HB-tag, Tandem-affinity purification, Ubiquitin-chain topology, Mass spectrometry
 Abstract: The covalent attachment of the small protein ubiquitin to other proteins is known to control a host of biological pathways and is emerging as an important regulatory factor in various processes specific to the nervous system. Ubiquitination is also tightly linked to most neurodegenerative disorders. A quantitative, proteome-wide view of the dynamic changes in ubiquitin modification associated with neuronal activity states and various stages of neurodegenerative disorders is therefore desired. Advances in quantitative mass spectrometry and the development of new biological tools make these approaches feasible for many laboratories. We describe here a combination of SILAC-based (stable isotope labeling by amino acids in cell culture) quantitative mass spectrometry and tandem-affinity purification to detect systemwide changes in ubiquitination and ubiquitin chain topologies that will be useful to probe the role of ubiquitin in the nervous system.

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Language(s): eng - English
 Dates: 2011-05-25
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1007/978-1-61779-111-6_15
 Degree: -

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Title: Neuroproteomics
Source Genre: Book
 Creator(s):
Li, Ka Wan1, Editor
Affiliations:
1 Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, VU University, Amsterdam, The Netherlands, ou_persistent22            
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISBN: 978-1-61779-110-9
DOI: 10.1007/978-1-61779-111-6

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Title: Neuromethods
Source Genre: Series
 Creator(s):
Walz, Wolfgang1, Editor
Affiliations:
1 University of Saskatchewan Saskatoon, SK, Canada, ou_persistent22            
Publ. Info: Humana Press
Pages: - Volume / Issue: 57 Sequence Number: - Start / End Page: 197 - 209 Identifier: ISSN: 0893-2336