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  Precision of Inhibition: Dendritic Inhibition by Individual GABAergic Synapses on Hippocampal Pyramidal Cells Is Confined in Space and Time

Müllner, F. E., Wierenga, C. J., & Bonhoeffer, T. (2015). Precision of Inhibition: Dendritic Inhibition by Individual GABAergic Synapses on Hippocampal Pyramidal Cells Is Confined in Space and Time. NEURON, 87(3), 576-589. doi:10.1016/j.neuron.2015.07.003.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-94B5-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-94B6-9
Genre: Journal Article

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 Creators:
Müllner, Fiona E.1, Author              
Wierenga, Corette J.1, Author              
Bonhoeffer, Tobias1, Author              
Affiliations:
1Department: Cellular and Systems Neurobiology / Bonhoeffer, MPI of Neurobiology, Max Planck Society, ou_1113545              

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Free keywords: CORTICAL INTERNEURONS; GABA(B) RECEPTORS; ACTION-POTENTIALS; CALCIUM-CHANNELS; CA2+ SPIKES; NEURONS; SPINES; PLASTICITY; INPUTS; COMPARTMENTALIZATION
 Abstract: Inhibition plays a fundamental role in controlling neuronal activity in the brain. While perisomatic inhibition has been studied in detail, the majority of inhibitory synapses are found on dendritic shafts and are less well characterized. Here, we combine paired patch-clamp recordings and two-photon Ca2+ imaging to quantify inhibition exerted by individual GABAergic contacts on hippocampal pyramidal cell dendrites. We observed that Ca2+ transients from back-propagating action potentials were significantly reduced during simultaneous activation of individual nearby inhibitory contacts. The inhibition of Ca2+ transients depended on the precise spike-timing (time constant < 5 ms) and declined steeply in the proximal and distal direction (length constants 23-28 mm). Notably, Ca2+ amplitudes in spines were inhibited to the same degree as in the shaft. Given the known anatomical distribution of inhibitory synapses, our data suggest that the collective inhibitory input to a pyramidal cell is sufficient to control Ca2+ levels across the entire dendritic arbor with micrometer and millisecond precision.

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Language(s): eng - English
 Dates: 2015
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: NEURON
Source Genre: Journal
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Publ. Info: 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA : CELL PRESS
Pages: - Volume / Issue: 87 (3) Sequence Number: - Start / End Page: 576 - 589 Identifier: ISSN: 0896-6273