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  Synaptotagmin-1 binds to PIP2-containing membrane but not to SNAREs at physiological ionic strength.

Park, Y., Seo, J. B., Fraind, A., Perez-Lara, A., Yavuz, H., Han, K., et al. (2015). Synaptotagmin-1 binds to PIP2-containing membrane but not to SNAREs at physiological ionic strength. Nature Structural and Molecular Biology, 22(10), 815-823. doi:10.1038/nsmb.3097.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-D663-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2062-3
Genre: Journal Article

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 Creators:
Park, Y.1, Author              
Seo, J. B., Author
Fraind, A., Author
Perez-Lara, A.1, Author              
Yavuz, H.1, Author              
Han, K., Author
Jung, S. R., Author
Kattan, I.2, Author              
Walla, P. J.2, Author              
Choi, M., Author
Cafiso, D. S., Author
Koh, D. S., Author
Jahn, R.1, Author              
Affiliations:
1Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society, ou_578595              
2Research Group of Biomolecular Spectroscopy and Single-Molecule Detection, MPI for biophysical chemistry, Max Planck Society, ou_578565              

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 Abstract: The Ca(2+) sensor synaptotagmin-1 is thought to trigger membrane fusion by binding to acidic membrane lipids and SNARE proteins. Previous work has shown that binding is mediated by electrostatic interactions that are sensitive to the ionic environment. However, the influence of divalent or polyvalent ions, at physiological concentrations, on synaptotagmin's binding to membranes or SNAREs has not been explored. Here we show that binding of rat synaptotagmin-1 to membranes containing phosphatidylinositol 4,5-bisphosphate (PIP2) is regulated by charge shielding caused by the presence of divalent cations. Surprisingly, polyvalent ions such as ATP and Mg(2+) completely abrogate synaptotagmin-1 binding to SNAREs regardless of the presence of Ca(2+). Altogether, our data indicate that at physiological ion concentrations Ca(2+)-dependent synaptotagmin-1 binding is confined to PIP2-containing membrane patches in the plasma membrane, suggesting that membrane interaction of synaptotagmin-1 rather than SNARE binding triggers exocytosis of vesicles.

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Language(s): eng - English
 Dates: 2015-09-212015-10
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nsmb.3097
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Title: Nature Structural and Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 22 (10) Sequence Number: - Start / End Page: 815 - 823 Identifier: -