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  Chapter 3 - Microscopy of Model Membranes: Understanding How Bcl-2 Proteins Mediate Apoptosis

Das, K. K., Unsay, J., & García-Sáez, A. J. (2015). Chapter 3 - Microscopy of Model Membranes: Understanding How Bcl-2 Proteins Mediate Apoptosis. In A. Iglic, C. V. Kulkarni, & M. Rappolt (Eds.), Advances in Planar Lipid Bilayers and Liposomes (pp. 63-97). Amsterdam: Elsevier. doi:10.1016/bs.adplan.2015.01.002.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0028-EA25-2 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002A-F3DD-5
Genre: Book Chapter

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http://www.sciencedirect.com/science/article/pii/S1554451615000034 (Abstract)
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Apoptosis is a form of programmed cell death that plays an important role in key biological processes like development of organisms, the correct functioning of the immune system, and the maintenance of the cellular homeostasis. Dysregulation in the apoptotic pathway leads to diseases like cancer or neurodegenerative disorders. The proteins of the B-cell lymphoma 2 (Bcl-2) family are key regulators of mitochondrial outer membrane permeabilization (MOMP) during apoptosis, which is a critical step in the cell's commitment to death. However, their mechanism of action is still under intense investigation. Here, we discuss how microscopy approaches applied to model membranes are used to understand the intrinsic apoptotic pathway involving MOMP. We describe how model membranes mimicking the outer mitochondrial membrane (LUVs, GUVs, SLB) are used to understand the mechanism of Bcl-2-mediated apoptosis using state-of-the-art techniques like atomic force microscopy and fluorescence correlation spectroscopy. These studies have revealed interesting features like the role of membrane in altering the affinity of Bcl-2 proteins, Bax and Bak proapoptotic activity, mechanistic differences between pro- and antiapoptotic members, and the critical helices involved in pore formation by Bax.
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 Creators:
Das, Kushal Kumar1, 2, Author           
Unsay, Joseph1, 3, Author           
García-Sáez, Ana Jesus1, 2, Author           
Affiliations:
1Max Planck Research Group Membrane Biophysics, Max Planck Institute for Intelligent Systems, Max Planck Society, ou_1497659              
2Interfaculty Institute for Biochemistry, University of Tübingen, Tübingen, Germany, ou_persistent22              
3German Cancer Research Center, Heidelberg, Germany, ou_persistent22              

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Free keywords: Abt. Spatz; Forschungsgruppe Garcia Saez
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 Dates: 2015-03-01
 Publication Status: Published online
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.1016/bs.adplan.2015.01.002
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Title: Advances in Planar Lipid Bilayers and Liposomes
Source Genre: Series
 Creator(s):
Iglic, Ales, Editor
Kulkarni , Chandrashekhar V. , Editor
Rappolt, Michael , Editor
Affiliations:
-
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 21 Sequence Number: - Start / End Page: 63 - 97 Identifier: ISBN: 978-0-12-802116-3