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  Toxoplasma gondii myosins B/C: one gene, two tails, two localizations and a role in parasite division

Delbac, F., Sänger, A., Neuhaus, E. M., Stratmann, R., Ajioka, J. W., Toursel, C., et al. (2001). Toxoplasma gondii myosins B/C: one gene, two tails, two localizations and a role in parasite division. The Journal of Cell Biology: JCB, 155(4), 613-623. doi:10.1083/jcb.200012116.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-E624-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-E625-F
Genre: Journal Article
Alternative Title : Toxoplasma gondii myosins B/C: one gene, two tails, two localizations and a role in parasite division

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JCellBiol_155_2001_613.pdf (Any fulltext), 691KB
 
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 Creators:
Delbac, Frédéric, Author
Sänger, Astrid, Author
Neuhaus, Eva Maria1, Author              
Stratmann, Rolf, Author
Ajioka, James W., Author
Toursel, Catherine, Author
Herm-Götz, Angelika, Author
Tomavo, Stanisla, Author
Soldati, Thierry2, Author              
Soldati, Dominique, Author
Affiliations:
1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: Apicomplexa; unconventional myosin XIV; localization; Toxoplasma gondii; cell division
 Abstract: In apicomplexan parasites, actin-disrupting drugs and the inhibitor of myosin heavy chain ATPase, 2,3-butanedione monoxime, have been shown to interfere with host cell invasion by inhibiting parasite gliding motility. We report here that the actomyosin system of Toxoplasma gondii also contributes to the process of cell division by ensuring accurate budding of daughter cells. T. gondii myosins B and C are encoded by alternatively spliced mRNAs and differ only in their COOH-terminal tails. MyoB and MyoC showed distinct subcellular localizations and dissimilar solubilities, which were conferred by their tails. MyoC is the first marker selectively concentrated at the anterior and posterior polar rings of the inner membrane complex, structures that play a key role in cell shape integrity during daughter cell biogenesis. When transiently expressed, MyoB, MyoC, as well as the common motor domain lacking the tail did not distribute evenly between daughter cells, suggesting some impairment in proper segregation. Stable overexpression of MyoB caused a significant defect in parasite cell division, leading to the formation of extensive residual bodies, a substantial delay in replication, and loss of acute virulence in mice. Altogether, these observations suggest that MyoB/C products play a role in proper daughter cell budding and separation.

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Language(s): eng - English
 Dates: 2001-09-282000-12-282001-10-052001-11-122001-11-12
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
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Title: The Journal of Cell Biology : JCB
  Other : J. Cell Biol.
Source Genre: Journal
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Publ. Info: New York, NY : Rockefeller Institute Press
Pages: - Volume / Issue: 155 (4) Sequence Number: - Start / End Page: 613 - 623 Identifier: ISSN: 0021-9525
CoNE: /journals/resource/991042742946024