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  The Dictyostelium Bcr/Abr-related protein DRG regulates both Rac- and Rab-dependent pathways

Knetsch, M. L. W., Schäfers, N., Horstmann, H., & Manstein, D. J. (2001). The Dictyostelium Bcr/Abr-related protein DRG regulates both Rac- and Rab-dependent pathways. EMBO Journal, 20(7), 1620-1629. doi:10.1093/emboj/20.7.1620.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-F0E6-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-F0E7-1
Genre: Journal Article
Other : A Bcr/Abr-related protein controls the organization of a tubolocisternal organelle, F-Actin dynamics, and cytokinesis in Dictyostelium

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EMBOJ_20_2001_1620.pdf (Any fulltext), 343KB
 
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 Creators:
Knetsch, Menno L. W., Author
Schäfers, Nicole, Author
Horstmann, Heinz1, 2, Author              
Manstein, Dietmar J.3, Author              
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
3Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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Free keywords: actin; contractile vacuole; Dictyostelium; RabD; Rac
 Abstract: Dictyostelium discoideum DdRacGap1 (DRG) contains both Rho-GEF and Rho-GAP domains, a feature it shares with mammalian Bcr and Abr. To elucidate the physiological role of this multifunctional protein, we characterized the enzymatic activity of recombinant DRG fragments in vitro, created DRG-null cells, and studied the function of the protein in vivo by analysing the phenotypic changes displayed by DRG-depleted cells and DRG-null cells complemented with DRG or DRG fragments. Our results show that DRG-GEF modulates F-actin dynamics and cAMP-induced F-actin formation via Rac1-dependent signalling pathways. DRG's RacE-GAP activity is required for proper cytokinesis to occur. Additionally, we provide evidence that the specificity of DRG is not limited to members of the Rho family of small GTPases. A recombinant DRG-GAP accelerates the GTP hydrolysis of RabD 30-fold in vitro and our complementation studies show that DRG-GAP activity is required for the RabD-dependent regulation of the contractile vacuole system in Dictyostelium.

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Language(s): eng - English
 Dates: 2000-11-212001-02-072001-04-02
 Publication Status: Published in print
 Pages: 10
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 Rev. Type: Peer
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Title: EMBO Journal
  Other : EMBO J.
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 20 (7) Sequence Number: - Start / End Page: 1620 - 1629 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061