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  Single-molecule tracking of myosins with genetically engineered amplifier domains

Ruff, C., Furch, M., Brenner, B., Manstein, D. J., & Meyhöfer, E. (2001). Single-molecule tracking of myosins with genetically engineered amplifier domains. Nature Structural and Molecular Biology, 8(3), 226-229. doi:10.1038/84962.

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Genre: Journal Article
Alternative Title : Single-molecule tracking of myosins with genetically engineered amplifier domains

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NatStructBiol_8_2001_226.pdf (Any fulltext), 574KB
 
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 Creators:
Ruff, Christine, Author
Furch, Marcus1, Author              
Brenner, Bernhard, Author
Manstein, Dietmar J.1, 2, Author              
Meyhöfer, Edgar, Author
Affiliations:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Dietmar Manstein Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497708              

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 Abstract: We combined protein engineering and single molecule measurements to directly record the step size of a series of myosin constructs with shortened and elongated artificial neck domains. Our results show that the step size has a clear linear dependence on the length of the neck domain and we also established that mechanical amplification in the myosin motor is based on a rotation of the neck domain relative to the actin-bound head. For all our constructs, including those with artificial necks, the magnitude of the neck rotation concurrent with the displacement step was approximately 30 degrees. The engineered change in the step size of myosin marks a significant advance in our ability to selectively modify the functional properties of molecular motors.

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Language(s): eng - English
 Dates: 2000-06-082000-12-012001-03-01
 Publication Status: Published in print
 Pages: 4
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 Rev. Type: Peer
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Title: Nature Structural and Molecular Biology
  Other : Nature Struct Biol
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 8 (3) Sequence Number: - Start / End Page: 226 - 229 Identifier: ISSN: 1545-9993
CoNE: https://pure.mpg.de/cone/journals/resource/954925603763