English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex.

Rigo, N., Sun, C., Fabrizio, P., Kastner, B., & Lührmann, R. (2015). Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex. EMBO Journal, 34(24), 3059-3073. doi:10.15252/embj.201592022.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-0A1B-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-7C68-0
Genre: Journal Article

Files

show Files
hide Files
:
2228223.pdf (Publisher version), 3MB
 
File Permalink:
-
Name:
2228223.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2228223_Suppl_1.pdf (Supplementary material), 2MB
Name:
2228223_Suppl_1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2228223_Suppl_2.pdf (Supplementary material), 853KB
Name:
2228223_Suppl_2.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Rigo, N.1, Author              
Sun, C.1, Author              
Fabrizio, P.1, Author              
Kastner, B.1, Author              
Lührmann, R.1, Author              
Affiliations:
1Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              

Content

show
hide
Free keywords: B complex; electron microscopy; immunolabelling; protein localisation; spliceosome
 Abstract: The spliceosome assembles on a pre-mRNA intron by binding of five snRNPs and numerous proteins, leading to the formation of the pre-catalytic B complex. While the general morphology of the B complex is known, the spatial arrangement of proteins and snRNP subunits within it remain to be elucidated. To shed light on the architecture of the yeast B complex, we immuno-labelled selected proteins and located them by negative-stain electron microscopy. The B complex exhibited a triangular shape with main body, head and neck domains. We located the U5 snRNP components Brr2 at the top and Prp8 and Snu114 in the centre of the main body. We found several U2 SF3a (Prp9 and Prp11) and SF3b (Hsh155 and Cus1) proteins in the head domain and two U4/U6 snRNP proteins (Prp3 and Lsm4) in the neck domain that connects the main body with the head. Thus, we could assign distinct domains of the B complex to the respective snRNPs and provide the first detailed picture of the subunit architecture and protein arrangements of the B complex.

Details

show
hide
Language(s): eng - English
 Dates: 2015-11-182015-12-14
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.15252/embj.201592022
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: EMBO Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 34 (24) Sequence Number: - Start / End Page: 3059 - 3073 Identifier: -