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  Structural Insight into the Complex of Ferredoxin and [FeFe] Hydrogenase from Chlamydomonas reinhardtii

Rumpel, S., Siebel, J. F., Diallo, M., Farès, C., Reijerse, E. J., & Lubitz, W. (2015). Structural Insight into the Complex of Ferredoxin and [FeFe] Hydrogenase from Chlamydomonas reinhardtii. ChemBioChem: A European Journal of Chemical Biology, 16(11), 1663-1669. doi:10.1002/cbic.201500130.

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 Creators:
Rumpel, Sigrun1, Author
Siebel, Judith F.1, Author
Diallo, Mamou1, Author
Farès, Christophe2, Author           
Reijerse, Edward J.1, Author
Lubitz, Wolfgang1, Author
Affiliations:
1Department for Biophysical Chemistry, Max-Planck-Institut für Chemische Energiekonversion, Stiftstrasse 34–36, 45470 Mülheim an der Ruhr (Germany), ou_persistent22              
2Service Department Farès (NMR), Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445623              

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Free keywords: electron transfer; hydrogenases; NMR spectroscopy; photosynthesis; protein-protein interactions
 Abstract: The transfer of photosynthetic electrons by the ferredoxin PetF to the [FeFe] hydrogenase HydA1 in the microalga Chlamydomonas reinhardtii is a key step in hydrogen production. Electron delivery requires a specific interaction between PetF and HydA1. However, because of the transient nature of the electron-transfer complex, a crystal structure remains elusive. Therefore, we performed protein-protein docking based on new experimental data from a solution NMR spectroscopy investigation of native and gallium-substituted PetF. This provides valuable information about residues crucial for complex formation and electron transfer. The derived complex model might help to pinpoint residue substitution targets for improved hydrogen production.

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Language(s): eng - English
 Dates: 2015-03-122015-05-252015-07-27
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/cbic.201500130
 Degree: -

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Title: ChemBioChem: A European Journal of Chemical Biology
  Other : ChemBioChem
Source Genre: Journal
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Publ. Info: Weinheim, Germany : Wiley-VCH
Pages: - Volume / Issue: 16 (11) Sequence Number: - Start / End Page: 1663 - 1669 Identifier: ISSN: 1439-4227
CoNE: https://pure.mpg.de/cone/journals/resource/110978984568897_1