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  Protein Cross-Linking and Oligomerization through Dityrosine Formation upon Exposure to Ozone

Kampf, C. J., Liu, F., Reinmuth-Selzle, K., Berkemeier, T., Meusel, H., Shiraiwa, M., et al. (2015). Protein Cross-Linking and Oligomerization through Dityrosine Formation upon Exposure to Ozone. Environmental Science & Technology, 49(18), 10859-10866. doi:10.1021/acs.est.5b02902.

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Kampf, Christopher J.1, Author              
Liu, Fobang1, Author              
Reinmuth-Selzle, Kathrin1, Author              
Berkemeier, Thomas1, Author              
Meusel, Hannah1, Author              
Shiraiwa, Manabu1, Author              
Pöschl, Ulrich1, Author              
Affiliations:
1Multiphase Chemistry, Max Planck Institute for Chemistry, Max Planck Society, ou_1826290              

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 Abstract: Air pollution is a potential driver for the increasing prevalence of allergic disease, and post-translational modification by air pollutants can enhance the allergenic potential of proteins. Here, the kinetics and mechanism of protein oligomerization upon ozone (O-3) exposure were studied in coated-wall flow tube experiments at environmentally relevant O-3 concentrations, relative humidities and protein phase states (amorphous solid, semisolid, and liquid). We observed the formation of protein dimers, trimers, and higher oligomers, and attribute the cross-linking to the formation of covalent intermolecular dityrosine species. The oligomerization proceeds fast on the surface of protein films. In the bulk material, reaction rates are limited by diffusion depending on phase state and humidity. From the experimental data, we derive a chemical mechanism and rate equations for a kinetic multilayer model of surface and bulk reaction enabling the prediction of oligomer formation. Increasing levels of tropospheric O-3 in the Anthropocene may promote the formation of protein oligomers with enhanced allergenicity and may thus contribute to the increasing prevalence of allergies.

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 Dates: 2015
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: ISI: 000361415800013
DOI: 10.1021/acs.est.5b02902
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Title: Environmental Science & Technology
Source Genre: Journal
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Publ. Info: Easton, PA : American Chemical Society
Pages: - Volume / Issue: 49 (18) Sequence Number: - Start / End Page: 10859 - 10866 Identifier: ISSN: 0013-936X
CoNE: https://pure.mpg.de/cone/journals/resource/954921342157