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  T-cell receptor repertoire of human peripheral CD161(hi)TRAV1-2(+) MAIT cells revealed by next generation sequencing and single cell analysis

Held, K., Beltran, E., Moser, M., Hohlfeld, R., & Dornmair, K. (2015). T-cell receptor repertoire of human peripheral CD161(hi)TRAV1-2(+) MAIT cells revealed by next generation sequencing and single cell analysis. HUMAN IMMUNOLOGY, 76(9), 607-614. doi:10.1016/j.humimm.2015.09.002.

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 Creators:
Held, Kathrin1, Author
Beltran, Eduardo1, Author
Moser, Markus2, Author              
Hohlfeld, Reinhard1, Author
Dornmair, Klaus1, Author
Affiliations:
1external, ou_persistent22              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

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Free keywords: VITAMIN-B METABOLITES; MOLECULAR-BASIS; ALPHA-CHAIN; MR1; HETEROGENEITY; RECOGNITION; DEFINES; CD161CD161; MAIT cells; T-cell receptor; T-cell receptor repertoire;
 Abstract: Mucosal-associated invariant T (MAIT) cells are a T-cell subset that expresses a conserved TRAV1-2 (V alpha 7.2) T-cell receptor (TCR) chain and the surface marker CD161. They are involved in the defence against microbes as they recognise small organic molecules of microbial origin that are presented by the non-classical MHC molecule 1 (MR1). MAIT cells express a semi-restricted TCR alpha chain with TRAV1-2 preferentially linked to TRAJ33, TRAJ12, or TRAJ20 which pairs with a limited set of beta chains. To investigate the TCR repertoire of human CD161(hi)TRAV1-2(+) T cells in depth we analysed the alpha and beta chains of this T-cell subset by next generation sequencing. Concomitantly we analysed 132 paired alpha and beta chains from single cells to assess the alpha beta pairing preferences. We found that the CD161(hi)TRAV1-2(+)TCR repertoire in addition to the typical MAIT TCRs further contains polyclonal elements reminiscent of classical alpha beta T cells. (C) 2015 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

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Language(s): eng - English
 Dates: 2015
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: HUMAN IMMUNOLOGY
Source Genre: Journal
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Publ. Info: 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA : ELSEVIER SCIENCE INC
Pages: - Volume / Issue: 76 (9) Sequence Number: - Start / End Page: 607 - 614 Identifier: ISSN: 0198-8859