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  Lighting up the cell surface with evanescent wave microscopy

Toomre, D., & Manstein, D. J. (2001). Lighting up the cell surface with evanescent wave microscopy. Trends in Cell Biology, 11(7), 298-303. doi:10.1016/S0962-8924(01)02027-X.

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Genre: Journal Article
Alternative Title : Lighting up the cell surface with evanescent wave microscopy

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TrendsCellBiol_11_2001_ 298.pdf (Any fulltext), 300KB
 
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 Creators:
Toomre, Derek1, Author              
Manstein, Dietmar J.1, 2, Author              
Affiliations:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Dietmar Manstein Group, Max Planck Institute for Medical Research, Max Planck Society, ou_1497708              

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Free keywords: Cell biology; Biochemistry; Techniques & Methods; green-fluorescent protein (GFP); microscopy; total internal reflection (TIR); method; technique; vesicle fusion; trafficking; exocytosis
 Abstract: Evanescent wave microscopy, also termed total internal reflection fluorescence microscopy (TIR-FM), has shed new light on important cellular processes taking place near the plasma membrane. For example, this technique can enable the direct observation of membrane fusion of synaptic vesicles and the movement of single molecules during signal transduction. There has been a recent surge in the popularity of this technique with the advent of green-fluorescent protein (GFP) as a fluorescent marker and new technical developments. These technical developments and some of the latest applications of TIR-FM are the subject of this review.

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Language(s): eng - English
 Dates: 2001-07-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Trends in Cell Biology
  Other : Trends Cell Biol.
Source Genre: Journal
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Publ. Info: Cambridge, UK : Elsevier Current Trends
Pages: - Volume / Issue: 11 (7) Sequence Number: - Start / End Page: 298 - 303 Identifier: ISSN: 0962-8924
CoNE: https://pure.mpg.de/cone/journals/resource/954925580131