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  OSO paradigm - a rapid behavioral screening method for acute psychosocial stress reactivity in mice

Brzózka, M. M., Unterbarnscheidt, T., Schwab, M. H., & Rossner, M. J. (2016). OSO paradigm - a rapid behavioral screening method for acute psychosocial stress reactivity in mice. Neuroscience, 314, 1-11. doi:10.1016/j.neuroscience.2015.11.043.

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1-s2.0-S0306452215010428-main.pdf (Publisher version), 499KB
 
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Brzózka, Magdalena M., Author
Unterbarnscheidt, Tilmann1, Author           
Schwab, Markus H.1, Author           
Rossner, Moritz J.1, Author           
Affiliations:
1Neurogenetics, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173664              

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Free keywords: OSO paradigm; Acute psychosocial stress; Social defeat; Psychiatric endophenotypes; Neuregulin-1 (Nrg1); Transcription factor 4 (Tcf4)
 Abstract: Chronic psychosocial stress is an important environmental risk factor for the development of psychiatric diseases. However, studying the impact of chronic psychosocial stress in mice is time consuming and thus not optimally suited to ‘screen’ increasing numbers of genetically manipulated mouse models for psychiatric endophenotypes. Moreover, many studies focus on restraint stress, a strong physical stressor with limited relevance for psychiatric disorders. Here, we describe a simple and a rapid method based on the resident–intruder paradigm to examine acute effects of mild psychosocial stress in mice. The OSO paradigm (open field – social defeat – open field) compares behavioral consequences on locomotor activity, anxiety and curiosity before and after exposure to acute social defeat stress. We first evaluated OSO in male C57Bl/6 wildtype mice where a single episode of social defeat reduced locomotor activity, increased anxiety and diminished exploratory behavior. Subsequently, we applied the OSO paradigm to mouse models of two schizophrenia (SZ) risk genes. Transgenic mice with neuronal overexpression of Neuregulin-1 (Nrg1) type III showed increased risk-taking behavior after acute stress exposure suggesting that NRG1 dysfunction is associated with altered affective behavior. In contrast, Tcf4 transgenic mice displayed a normal stress response which is in line with the postulated predominant contribution of TCF4 to cognitive deficits of SZ. In conclusion, the OSO paradigm allows for rapid screening of selected psychosocial stress-induced behavioral endophenotypes in mouse models of psychiatric diseases.

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Language(s): eng - English
 Dates: 2015-11-252016-02
 Publication Status: Issued
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 Table of Contents: -
 Rev. Type: Peer
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Title: Neuroscience
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 314 Sequence Number: - Start / End Page: 1 - 11 Identifier: ISSN: 0306-4522
CoNE: https://pure.mpg.de/cone/journals/resource/954925514498